OXIDATIVE STRESS AND RESISTANCE OF ERYTHROCYTES MEMBRANES IN PATIENTS WITH CHRONIC KIDNEY DISEASE STAGE VD DEPENDING ON MODALITY OF RENAL REPLACEMENT THERAPY
Journal Title: УКРАЇНСЬКИЙ ЖУРНАЛ НЕФРОЛОГІЇ ТА ДІАЛІЗУ - Year 2016, Vol 2, Issue 50
Abstract
The aim of the research was to study the effect ofoxidative factors impact and modality of renal replacement therapy (BBT) on indices ofoxidative stress (OS) and resistance of erythrocytes membranes in patients with chronic kidney disease stage V(CKD VD) and anemie. Material and methods. The study involved 68 patients with CKD VD: 14 patients were treated by hemodiafiltration (HDF), 25 patients by hemodialysis (HD) and 29 patients by peritoneal dialysis (PD). The severity ofanemia was assessed according to the KDIGO (2012) criteria. The control group consisted of 30 healthy people of the same age and sex. Along with the standard diagnostic methods, we defined the content of malonic dialdehyde in serum (MDAs) and in erythrocytes (MDAe), the content of ceruloplasmin (CPs), transferrin (TBs) and SH-groups in the blood serum, the index of the OS (IOS), catalase activity in serum (CATs), glucose-6-phosphate dehydrogenase (G-6-PDHe) and total peroxidase activity (TPA) in erythrocyte, peroxide resistance (PR) of red blood cells and erythrocyte membrane permeability (EMP). Statistical analysis was performed using the programs of Microsoft Excel 7.0. Results. It has been stated that in the CKD VD patients in compatison with control group the MDAs content increased by 3.3 times and MDAe - 1.2 times, TBs content reduced by 34%, SH-groups - by 31%, TPAe - by 41% and G- 6-FDGe - by 58%, PB-by 60%; 4.6 times increased CATs activity and OSI; 2 times grew peroxide hemolysis (PH) and 1.3 times - EMP. The analysis (depending on the BBT modality) showed that the patients treated by HDF had typical MDAs increase by 3.9 times on a background of CPs by 24%, TBs - 33%, SH-groups - 25%, TPAe - 51%, G-6-PDHe - 42%; the increase in serum OSI - 5.4 times and 2.6 times in erythrocytes, PB - by 3.6 times and CATs activity by 3.5 times; HD group was characterized by the highest value of MDAe, OSI, PH and CATs, along with more expressed decrease of TBs indices, SH-groups, TPA and G-6-FDHe activity compared with rates in patients with HDF. The patients treated with PD had the lowest content of MDAs and the highest values on the background ofTPAe, the significant increase of CPs by 1.7 times and lowest TBs and G-6-PDHe. The patients with PD showed twice lower OS activity by OSI. Conclusion. Thus, in patients with CKD VD, who had HD, HDF or PD an anemie was associated with high OS activity and the increased degree of hemolysis. These changes are stipulated by BBT methods: for patients receiving HDF were typical the lowest rates of hemolysis and the highest degree ofprotection for erythrocytes, and for patients treated with HD - the highest OS.
Authors and Affiliations
L. V. Korol, L. Ya. Migal, O. Burdeyna, M. Kolesnyk
Keywords
Related Articles
- EP ID EP354373
- DOI 10.31450/ukrjnd.2(50).2016.01
- Views 65
- Downloads 0
PREDICTION OF UNFAVORABLE COURSE OF NEPHROTIC SYNDROME IN CHILDREN: THE MULTIMARKER PARALLEL CASCADE SYSTEM
The aim of study was to investigate the possibility of using the results of different diagnostic tests in children with nephrotic syndrome (NS) asprognostic markers during disease course. Materials and methods. The oper...
CYTOKINE IMMUNITYINCKD PATIENTS TREATED WITH HD, HDF, PD
Introduction. Cytokines play the corresponding role in the inflammatory processes and the development of endothelial dysfunction and therefore take an important place in the problem of systemic chronic inflammation (SCI)...
Peritoneal dialysis - associated peritonitis during treatment with continuous ambulatory peritoneal dialysis
Infections associated with peritoneal dialysis (infection of the catheter, tunnel infection and peritonitis) are the most common complications of this method. Despite significant progress in the methodological approaches...
ESTIMATION OF NEPHROPROTECTIVE EFFECT OF ANGIOTENSIN-CONVERTING ENZYME RAMIPRIL IN PATIENTS WITH CHRONIC GLOMERULONEPHRITIS
Цель: изучить взаимосвязь между морфологическими показателями гломерулярного поражения почек и цистатином С (CysC) сыворотки крови у больных хроническим гломерулонефритом (ХТН) с сохраненной функцией почек, а также оцени...
ПРАКТИЧНА ЦІННІСТЬ ВИЗНАЧЕННЯ ПОКАЗНИКІВ ОКСИДАТИВНОГО СТРЕСУ У ХВОРИХ НАПІЄЛОНЕФРИТ
Робота присвячена практичному застосуванню показників оксидативного стресу в клінічній практиці та використання їх для моніторингу та оптимізаціїлікування хворих на пієлонефрит.