OXIDATIVE STRESS, ANTIOXIDANT STATUS AND 5’-NUCLEOTIDASE ACTIVITY IN CHRONIC HEPATITIS C AND ALCOHOLIC PATIENTS
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2015, Vol 4, Issue 1
Abstract
Background: The present study was undertaken to estimate the 5’-nucleotidase enzyme in chronic hepatitis C and alcoholics. The oxidative stress, antioxidants and their correlation with 5’-nucleotidase was also included in this study. Methods: The blood samples of 25 subjects (age and sex matched) each from group I (control), group II (alcoholic) and group III (chronic hepatitis C) was taken and centrifuged for separation of plasma for analysis of 5’-nucleotidase. The separated cells were washed thrice with 0.9 % w/v cold normal saline and used for the analysis of glutathione, malondialdehyde and superoxide dismutase. Results: The activity of serum 5’- nucleotidase was significantly increased in both chronic hepatitis C and alcoholics. The levels of malondialdehyde were also significantly increased in both chronic hepatitis C and alcoholic patients. The levels of glutathion and superoxide dismutase were significantly decreased in both chronic hepatitis C and alcoholics. Conclusions: From these findings it was concluded that the activity of serum 5’- nucleotidase rises consistently in chronic hepatitis C and alcoholics according to the extent of liver damage, hepatobiliary damage, and biliary stasis and can be a useful marker for diagnosis of hepatobiliay disorders.
Authors and Affiliations
Tarannum Fatima Subhani| Department of Biochemistry, HIMS, Sitapur, India, Md. Abu Nasar| Department of Biochemistry, Nalanda Medical College, Patna, India, R. P Gupta| Department of Biochemistry, Nalanda Medical College, Patna, India, Md. Arif Naseer| Department of Pharmaceutical Chemistry, Adarsh Vijendra Institute of Pharmaceutical Sciences, School of Pharmacy, Shobhit University, Gangoh, Saharanpur, India
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