Oxidative Stress Biomarkers and Their Relationship with Testosterone in Male Auto Mechanics in Ibadan, Nigeria
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 12, Issue 9
Abstract
Occupational exposure to mixed chemicals generates free radicals with inadequate antioxidants resulting in oxidative stress. Recently, hypogonadism in male auto-mechanics was associated with oxidative stress. Studies show that testosterone, a male hormone increases the activities of antioxidant enzymes. This study is aimed at evaluating the oxidative stress biomarkers and their relationship with testosterone in auto mechanics in Ibadan, Nigeria. Eighty-three males participated in this prospective cross sectional study after informed consent. Forty-three were male auto-mechanics, occupationally exposed to mixed chemicals in the mechanic community, Bodija, Ibadan (cases). Their mean (SEM) age and body mass index (BMI) were 42.5 (1.7) years and 23.8 (0.5) Kg/m2 respectively. They were age and BMI matched with 40 unexposed, apparently healthy males from the University College Hospital and environs (controls). Demography, social habits, anthropometry and gonadal status were obtained by standard methods. Serum obtained from blood (10 ml) collected from the participants was used for biochemical analyses. Testosterone levels were determined by enzyme immunoassay method (Immunometrics UK Ltd). Levels of total antioxidant capacity, total plasma peroxide (TPP), malondialdehyde (MDA), hydrogen peroxide (H2O2), glutathione peroxidase (GPX), superoxide dismutase (SOD), glutathione-S-transferase (GST), and reduced glutathione (GSH) were determined using spectrophotometric methods while oxidative stress index (OSI) was calculated. P<0.05 was regarded as significant. TPP, MDA, OSI, H2O2 and GST `levels were significantly higher (P<0.001) in eugonadal cases compared with controls. All these biomarkers levels were similar in hypogonadal compared with eugonadal cases. (P>0.05) Testosterone related negatively with SOD in the controls only but positively with MDA and negatively with GST in cases only (P<0.05). Occupationally exposed auto mechanics appear to have oxidative stress and may benefit improvement in antioxidant status. Testosterone may contribute to and enhance total antioxidant status, which may be important in gonadal function.
Authors and Affiliations
S. A. Adekola, M. A. Charles-Davies
Keywords
Related Articles
- EP ID EP340431
- DOI 10.9734/BJMMR/2016/22350
- Views 48
- Downloads 0
Investigation of Dynamic Thiol-Disulfide Homeostasis in Alopecia Areata Patients
Background: Thiols are antioxidant, organic compounds containing sulfhydryl group on their active sites. They have important roles on preventing oxidative stress. The thiol disulfide homeostasis has a vital importance in...
Occupational Risk Factors for Musculoskeletal Disorders among Operation Room Nurses at Cairo University Hospitals
Aims: To assess the occupational risk factors that contribute to the occurrence of work- related musculoskeletal disorders among Operation Room (OR) nurses and MSDs relation to biomarker c-telopeptide of type II collag...
Patient Satisfaction with Services at a General Outpatient Clinic of a Tertiary Hospital in Nigeria
Aims: To determine the factors influencing patient satisfaction with services at the general outpatient clinic of a tertiary hospital in Nigeria. Study Design: A descriptive cross sectional study. Place and Duration of...
Putative Risk Factors among Ghanaian Women Presenting with Leiomyoma
Aims: The objective of the current study was to investigate potential demographic, lifestyle, and medical history risk factors for leiomyoma in a sample of Ghanaian women. Study Design: A case-control study was conducted...
Mild Hypothermia Attenuates the Excitatory Amino Acids Overflow and Diminishes the Nitric Oxide Synthase Activity during Reperfusion after Asphyxial Cardiac Arrest in Rats
Aims: Neurotransmitter overflow into the extracellular space and activation of nitric oxide synthase were implicated in neuronal death after cerebral ischemia. A small temperature reduction induced after the insult cruci...