OXYGRAPHIC EVALUATION OF ACTIVITY OF RESPIRATORY ENZYMES IN BOAR'S SEMEN
Journal Title: Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica - Year 2016, Vol 40, Issue 330
Abstract
In the article advantages of the use of oxygraphic method for metabolic examination of mitochondrial respiratory enzymes in boar spermatozoa were described. Investigations were performed on semen samples taken from 36 boars of 4 breeds, at the age from 8 to 40 months. All samples of semen characterized at least 80% content of motile spermatozoa. The rate of oxygen uptake was measured with the use of Clark type electrode connected with analog-digital recorder. Measurements were executed in temperature 37°C, and the rate of oxygen uptake (v) was expressed in nmol O2 · min–1 per 108 spermatozoa. The values of oxygen consumption rate after application of succinate (vs) and PMS (vp) made up the basis of analysis. The results show that the method of oxygraphic evaluation of spermatozoa movement system described in the paper makes possible the precise investigation of the spermatozoa respiratory chain status and enables selection of boars with the best parameters of semen. This method can be taken in consideration at semen examination in the course of boar's selection for the AI stations, after boar's reproductive disease, in periodical semen tests and also when taking decision about the boar's elimination.
Authors and Affiliations
Piotr KOPCZYŃSKI, Bogdan LASOTA, Tadeusz OGOŃSKI, Tomasz STANKIEWICZ, Dariusz GĄCZARZEWICZ, Magdalena SKOLMOWSKA
Keywords
Related Articles
- EP ID EP411789
- DOI -
- Views 80
- Downloads 0
BEHAVIOUR OF CALVES
The activity of calves depends mainly on the way they are housed and not on their age. Because compromised welfare early in life may affect the profitability of future production, calves need to be fed colostrum after bi...
MALOPOLSKI HORSES – SPORT OR RECREATION?
Many horse breeders currently face the problem of breeding which is oriented towards the purposes of sport, which places increasing demands on riding horses. But would it not be more rational to plan a strategy focused p...
SOIL CONDITIONS AND XEROTHERMIC GRASSES COMMUNITIES WITHIN THE WESTERN EDGE OF THE ODER IN THE VICINITY OF SZCZECIN
Based on 29 relevés were made in the area of the edge of Western Oder (Ustowo– –Pargowo) two xerothermic grass associations were distinguished. Potentillo-Stipetum capillatae phytocoenon is internally differentiated into...
SEGETAL FLORA OF THE PLANTATIONS OF VIRGINIA FANPETALS SIDA HERMAPHRODITA (L.) RUSBY IN ŁOBEZ COMMUNE (WEST POMERANIA)
This paper presents the results of the investigation of the segetal flora accompanying the Virginia fanpetals crops in Łobez Commune. The purpose of this research was the identification and ecological analysis of weeds r...
ANALYSIS OF THE EFFECT OF THE ORDER OF CALVING, CALF SEX AND MILK YIELD OF COWS ON GROWTH PARAMETERS OF LIMOUSIN CALVES
The study was taken in the farm located in West Pomerania province where Limousine cattle in purity of breed were kept. The study included a total of 226 Limousine calves in purity of breed, which were born between 2011...