Panton-Valentine Leukocidin gene positive methicillin resistant Staphylococcus aureus, the community strains causing infections in intensive care unit - High risk of outbreak and preventing strategies
Journal Title: Journal of Medical and Allied Sciences - Year 2016, Vol 6, Issue 2
Abstract
Methicillin resistant Staphylococcus aureus (MRSA) is an important pathogen that causes hospital acquired infections recorded in the intensive care unit (ICU). Most of the MRSA isolates carry mecA gene which is a molecular marker for methicilin resistance. There are two types of MRSA, community acquired (C–MRSA) and hospital acquired (H-MRSA), both of these contain mecA gene. The Panton-Valentine Leukocidin (PVL) gene is normally present in C-MRSA infections which are now found to be widespread in hospital setting. Our objective was to assess the presence of PVL gene in mecA gene positive MRSA isolates from ICUs. This was a cross sectional study in ICUs of a tertiary care hospital over a period of 8 months (June 2013 to January 2014). Total of two hundred patients admitted in the ICUs who were suspected to have acquired infection 48 hours after admission were included in the study. By routine bacteriological examination and disc diffusion sensitivity testing with 30μg cefoxitin discs, MRSA strains were isolated. Polymerized chain reaction (PCR) was performed to detect mecA gene and PVL gene using specific primers. Cefoxitin disc diffusion screening showed 112 positive MRSA strains among which the mecA gene was detected in 104 strains and was absent in the remaining eight strains of total MRSA. Among the 104 mecA gene positive MRSA strains, 46 (44%) strains contained PVL gene. Our results indicate a higher prevalence of PVL-positive MRSA strains in the ICUs compared to many earlier studies. These strains were susceptible only to very few antibiotics and the empirical treatment options should be planned accordingly. Awareness of intensive care physicians and proper training of health care workers in the ICU could lower the magnitude of this problem.
Authors and Affiliations
Mowna Karthik| Department of Microbiology and Vinayaka Mission's Kirupananda Variyar Medical College, Salem-636308, Tamil Nadu, India, Prabhu Thilaak| Department of Anesthesiology & Intensive Care, Vinayaka Mission's Kirupananda Variyar Medical College, Salem-636308, Tamil Nadu, India, Indra Priyadharsini| Department of Microbiology and Vinayaka Mission's Kirupananda Variyar Medical College, Salem-636308, Tamil Nadu, India, Senthil Marappan| Department of Anesthesiology & Intensive Care, Vinayaka Mission's Kirupananda Variyar Medical College, Salem-636308, Tamil Nadu, India
Keywords
Related Articles
- EP ID EP12744
- DOI 10.5455/jmas.222450
- Views 332
- Downloads 26
Homology modeling and ligand interaction of Cytochrome b protein
Cytochrome b is a component of respiratory chain complex III, also known as the bc1 complex or ubiquinol-cytochrome c reductase. These complexes are involved in electron transport and the generation of ATP and thus pla...
Medical humanities
An interesting case of self-inflicted abdominal injury with multiple transections of the bowel
We present an unusual case of self-inflicted abdominal injury where the patient had used a razor blade to give himself a laparotomy-like incision and also brought a piece of excised and charred bowel with him to the ho...
Pulmonary tuberculosis: A comparative study of conventional methods and serological diagnosis
The objective of the study was to evaluate mycobacterial identification and isolation rates obtained by different conventional methods. Sputum and serum samples were taken from 80 clinically diagnosed pulmonary tubercul...
Periocular anthropometric study among adult Bangladeshi Buddhist Chakma females
The dimensions of the human body are affected by geographical, racial, ethnic, gender and age factors. So, a normative data of periocular measurements based on above factors are indispensable to identify any ocular pat...