PARAMETERS OPTIMIZATION FOR INCREASED INTRACELLULAR INULINASE ACTIVITY OF A YEAST STRAIN
Journal Title: Ecological Engineering and Environment Protection (EEEP) - Year 2019, Vol 1, Issue 1
Abstract
This study reveals the selection of a yeast strain, possessing inulinase activity and finding the optimal conditions of cultivation. Intra- and extracellular activity assay was performed after cultivation on media, containing inulin as a sole source of carbon. Optimization of the cultivation conditions was carried out for establishing the favorable conditions for biosynthesis of inulinase. Modifying the physicochemical and nutritional parameters of a cultivation process lead to major improvement of the enzyme activity. Highest intra- and extracellular inulinase activity was registered when 1.5% inulin was used, 5 % inoculum, temperature 28°C, pH=6.5 and agitation of 200rpm. The selected strain Kluyveromyces sp. C showed higher values for the intracellular inulinase activity, making it suitable for immobilization and further use.
Authors and Affiliations
Lyudmila Kabaivanova, Adriana Goushterova, Petar Grozdanov, Elena Chorukova
Keywords
Related Articles
- EP ID EP636009
- DOI 10.32006/eeep.2019.1.6270
- Views 84
- Downloads 0
EVALUATION OF COMPOSTING MATERIALS FOR SUSTAINABLE AGRICULTURAL APPLICATIONS
. Natural resources are elements of the environment that humans use as a means of production in order to achieve economic development. The aim of the study is based on the evaluation of the proposed 5 natural products /v...
CURRENT SITUATION AND SUSTAINABLE DEVELOPMENT OF WATER RESOURCES IN SENEGAL
Water is essential for human life, the socio-economic improvement of the state and the protection of its natural environment. Senegal has a rich and diversified hydrological potential, most of whose surface water reserve...
FIRE SEVERITY ASSESSMENT USING NDVI DERIVED FROM LANDSAT TM/ETM IMAGES AND TERRAIN DATA
The forest fires influence on the plants and soil depends on the fire severity and time of exposure. Fire severity integrates physical, chemical and biological changes occurring in ecosystems on the area as a consequence...
PATHOGENIC BACTERIA IN WATERS AND DRINKING WATER-ASSOCIATED BIOFILMS
In this review, the dissemination of bacterial pathogens in natural waters and the mechanisms of their transmission in drinking water, and the role of water-associated biofilms for their survival or growth are discussed....
ASPECTS AND PERSPECTIVE OF INTERIM ECOLOGICAL MONITORING APPLICATION ON ECOSYSTEMS BY MEANS OF REMOTE SENSING
Identifying and detailing the biophysical characteristics of species and habitats, habitat mapping, predicting the distribution of species and spatial variability in species richness and detecting the influence of natura...