PARAMETERS OPTIMIZATION FOR INCREASED INTRACELLULAR INULINASE ACTIVITY OF A YEAST STRAIN
Journal Title: Ecological Engineering and Environment Protection (EEEP) - Year 2019, Vol 1, Issue 1
Abstract
This study reveals the selection of a yeast strain, possessing inulinase activity and finding the optimal conditions of cultivation. Intra- and extracellular activity assay was performed after cultivation on media, containing inulin as a sole source of carbon. Optimization of the cultivation conditions was carried out for establishing the favorable conditions for biosynthesis of inulinase. Modifying the physicochemical and nutritional parameters of a cultivation process lead to major improvement of the enzyme activity. Highest intra- and extracellular inulinase activity was registered when 1.5% inulin was used, 5 % inoculum, temperature 28°C, pH=6.5 and agitation of 200rpm. The selected strain Kluyveromyces sp. C showed higher values for the intracellular inulinase activity, making it suitable for immobilization and further use.
Authors and Affiliations
Lyudmila Kabaivanova, Adriana Goushterova, Petar Grozdanov, Elena Chorukova
Keywords
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- EP ID EP636009
- DOI 10.32006/eeep.2019.1.6270
- Views 65
- Downloads 0
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