Partial purification and characterisation of polyphenol oxidase from tomatoes (solanum lycopersicum)
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2013, Vol 1, Issue 6
Abstract
Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the substrates tested, pyrogallol was the best substrate for PPO with a Km value of 1.5 mM. The optimum pH for PPO activity was found to be 6.8. The enzyme showed high activity over a broad pH range of 4 - 8. The optimal pH and temperature for enzyme activity were found to be 6.8 and 50-60 °C, respectively. km value for tomatoe PPO is calculated 25 mM for catechol and 1.5 mM for pyrogallol and 8.5 mM for L-dopa. As can be seen, affinity of PPOs for various substrates varies widely. The enzyme showed a broad activity over a broad pH and temperature range. The thermal inactivation studies showed that the enzyme is heat resistant. The enzyme showed the highest activity toward pyrogallol and no activity toward tyrosine. Of the inhibitors tested, the most potent inhibitors was sodium kojic acid.
Authors and Affiliations
Shahryar saeidian| Assistan professor of biochemistry, Department of Biology, Payame noor university, Iran, E-mail: saeedyan@pnu.ac.ir
Keywords
Related Articles
- EP ID EP12846
- DOI -
- Views 379
- Downloads 19
The Role of Economic Development and Management in Agriculture: a Brief Review
Agriculture has a key role to play in reducing poverty and hunger in many developing countries. About 75% of the world's poor live in rural areas and most are dependent on agriculture and related activities in the rura...
Examine the effects of training on personality formation and mental development of childhood and adolescence
The present study was conducted to evaluate the effect of training on personality formation and mental development of childhood and adolescence. It is important to understand how children develop physically, socially,...
Antimicrobial Impact of Hibiscus Sabdariffal Extract Against Acinetobacterbaumannii
Objective: Antibiotic resistant to Antimicrobial agent is one of the most important concern in hospitals, which can cause lots of costs, treatment fails and mortality rates. Acinetobacter baumannii is a Gram-negative b...
Evaluation of Crategus sp. spatial pattern in the Central Zagros Forest
Spatial pattern of trees can possibly indicate stand history, population dynamics, and species competition. This research was investigated in the Chahartagh forest reserve, Ardal region, chaharmehal and Bakhtiari Provi...
Analysis Students’ Motivation in Vocational Schools and Agricultural Training Centers in Kermanshah Province toward Studying the Field of Agriculture
Objective: Today people are aware of the important role of agriculture as a vital source in their life. Development in agriculture and making healthy food for human, needs trained man. Vocational schools and agricultur...