Partial Purification of Cellulase Enzyme from Fusarioum oxysporium by Using Egg White Matrix
Journal Title: Biotechnology Journal International - Year 2014, Vol 4, Issue 2
Abstract
Aim: Protein matrix possesses a capacity to bind organic and inorganic matter. Egg white matrix as an adsorbent was utilized for partial purification of cellulase enzyme in detailed batch study. Methodology: Effect of pH, matrix size, enzyme concentration, contact time and temperature on the binding of cellulase on egg white matrix was studied. Desorption studies was carried with variable pH, sodium chloride and ammonium sulphate system to desorbs the cellulase for the egg white matrix. Results: Equilibrium isotherms for the adsorption of the cellulase enzyme on egg white matrix were well fitted to Freundlich and Langmuir isotherm models. The adsorption process has been found endothermic in nature and thermodynamic parameters, Gibb’s free energy (∆Gº), change in enthalpy (∆Hº) and change in entropy (∆Sº) have been calculated as -13276.21, 2025.47 and 698.64 (KJ mol-1) respectively. Thermodynamic studies revealed that the adsorption process is spontaneous and endothermic in nature. In desorption studies it was concluded that maximum elution (66.8% was obtained for cellulase enzyme with 0.3M (NH4)2SO4 with respect to different pH and sodium chloride solution. Cellulase enzyme obtained after desorption process has maximum purification fold of 4.25 with 60% recovery. Conclusion: The results from the current research suggest that egg white can be used for partial purification of cellulase enzyme with less cost.
Authors and Affiliations
Jagdish Singh, Manpreet Kaur
Keywords
Related Articles
- EP ID EP237187
- DOI 10.9734/BBJ/2014/6772
- Views 122
- Downloads 0
Antifungal Activity of Camel Faeces with Special Reference to Dermatophytes
Aim: The present study was conducted to evaluate the antifungal activity of camel faeces on some pathogenic fungi. Study Design: This is a descriptive evaluation study. Methodology: Camel faeces was extracted following H...
Genetic Variations in Asparagus racemosus, an Endangered Medicinal Herb Endemic to India Using RAPD Markers
Aim: To study the genetic diversity in Asparagus racemosus germplasm using RAPD molecular markers for its better conservation and utilization. Study Design: RAPD markers used to check genetic diversity in Asparagus racem...
Antimicrobial Resistance and Detection of Biofilm in Staphylococcus aureus Isolates from Casablanca
Aims: The ability of biofilm formation seems to play an essential role in the virulence of Staphylococcus aureus. The aims of the present study were to test the sensitivity of the clinical isolates of Staphylococcus aure...
Listeria spp. in Raw Cow and Goat Meat in Port Harcourt, Nigeria
Aims: To determine the occurrence of Listeria monocytogenes and other Listeria spp. in raw cow and goat meat. Study Design: This work was based on a completely randomized design with two replication and the average value...
Optimization of Polyphenols Extraction Method from Kola Nuts (Cola nitida Vent. Schott & Endl.) Using Experimental Design
Aims: The aim of this study is to optimize extraction process of phenolics compounds from kola nuts by using experimental design. Study Design: Kola nuts were collected in October 2014-February 2015 in south of Côte d’Iv...