Pathogenetic Significance of Antimicrobial Peptides in the Implementation of Antibacterial Protection in Children with Cystic Fibrosis
Journal Title: Здоров`я дитини - Year 2013, Vol 3, Issue 46
Abstract
Objective: to investigate the factors increasing the colonization of respiratory system with Ps.аeruginosa in children with cystic fibrosis. Materials and methods. The study involved 37 children with cystic fibrosis at the age from 3 to 15 years. The control group consisted of 30 healthy children. The results of the bacteriological examination of sputum were evaluated. The plasma levels of α-defensins 1–3 (HNP 1–3), serum levels of C-reactive protein (CRP), metabolites of nitric oxide (NO), inducible NO-synthase (iNOS), NTproBNP were investigated. Results. According to the results of bacteriological examination of sputum pathogens was identified in 22 (59.4 %) patients. The dominant microorganisms were presented by Ps.aeruginosa in 14 (37.8 %) patients. Patients with cystic fibrosis have had 3.9 times increased levels of HNP 1–3 in plasma in comparison with control group (p < 0.05). The presence of Ps.aeruginosa led to the 6.8 times increase of HNP 1–3 in plasma of children with cystic fibrosis (p < 0.001). Simultaneously, the presence of Ps.aeruginosa resulted in an increase of CRP (p < 0.01). Contamination of the patients with cystic fibrosis by Ps.aeruginosa contributed to lack of NO and iNOS production. Colonization of the lower respiratory tract by Ps.aeruginosa associated with 1.7 times increased levels of NTproBNP in comparison with control group (p < 0.05). Conclusions: 1. Children with cystic fibrosis had been characterized by elevation of plasma level of α-defensins 1–3. Production of HNP 1–3 adaptively increased in response to the contamination of the respiratory tract by Ps.aeruginosa, but high concentration of α-defensins 1–3 depressed phagocytic activity of neutrophils. Under such conditions compensatory-adaptive response, that was intended at the overcoming of pathogenic contamination, take a turn of pathologic and was an additional factor of respiratory system affection. 2. In children with cystic fibrosis reduction of generation of nitric oxide regardless of the microbial status of respiratory system took place. Availability of Ps.аeruginosa in respiratory system microbiota assisted to the adequate course of the second phase of inflammation at the expense of absence of іNOS adequate activation. 3. Increased serum level of NTproBNP in children with cystic fibrosis, infected with Ps.aeruginosa, is a compensatory reaction in response to the activation of endothelin-1 synthesis and serves as additional factor of supporting of chronic inflammation and damage of the respiratory system.
Authors and Affiliations
G. O. Lezhenko, O. Ye. Abaturov, L. I. Pantyushenko
Keywords
Related Articles
- EP ID EP234447
- DOI -
- Views 91
- Downloads 0
Gastritol in Correction of Clinical Symptoms and Disorders of Gastric Secretion in Functional Dyspepsia in Children
The article presents the diagnostic criteria for functional dyspepsia, postprandial syndrome, epigastric pain syndrome. The findings on Gastritol effectiveness in the correction of clinical symptoms of functional dyspeps...
Evaluation of the Immune System State in Patients with Chemoresistant Pulmonary Tuberculosis Depending on the Clinical Form
The objective of the work was to assess the state of the immune system in patients with chemoresistant pulmonary tuberculosis, depending on the clinical form in terms of cytokines, leukocyte counts and haematological ind...
The dynamics of the health of schoolchildren in Ukraine according to the questionnaire survey
Background. For the last decades, the state of health and physical development of the children’s population of Ukraine are deteriorating. Among the factors that determine the decline in children’s health, educational loa...
Role of the Gut Microbiota in the Development of Lipid and Carbohydrate Metabolism in the Liver Steatosis in Children
Background. Nonalcoholic fatty liver disease (NAFLD) is associated with insulin resistance, dyslipidemia and obesity. Recent evidence supports a role of the gut microbiota in the pathogenesis of NAFLD. Changes in the mi...
Screening Epidemiological Characteristics of Blood Pressure in Schoolchildren from Ivano-Frankivsk
The article presents the results of the epidemiological study carried out in the group of 1352 schoolchildren aged 12–17 years from Ivano-Frankivsk. Parameters of systolic and diastolic blood pressure and the ranges of f...