Pathogenic spectrum and molecular characteristics of infectious diarrhea among children in Putuo District, Shanghai
Journal Title: Shanghai Journal of Preventive Medicine - Year 2024, Vol 36, Issue 6
Abstract
[Objective] To investigate the pathogenic spectrum and molecular characteristics of infectious diarrhea among children in Putuo District of Shanghai from 2018 to 2023, and to provide scientific basis for the prevention and control of infectious diarrhea in children. [Methods] Fecal samples from the cases visited sentinel hospitals for children’s diarrheal disease in Putuo District, Shanghai, were collected from January 2018 to December 2023. A total of 11 species of bacteria were isolated and cultured, and 5 species of viruses were detected by real-time fluorescent polymerase chain reaction (PCR). The molecular typing of some positive strains was analyzed by the standard pulsed-field gel electrophoresis (PFGE) method. The polymerase-capsid protein linkage region of some norovirus-positive samples was amplified by reverse transcription PCR, and was sequenced and analyzed by bioinformatics software. The Chi-square test and Fisher’s exact probability test were used to compare the pathogen-positive rates in 2018‒2019 and 2020‒2023. [Results] Of the 707 cases of children with infectious diarrhea, the total positive rate was 47.67%, with a single bacterial positivity rate of 16.27%, a single viral positivity rate of 22.63%, and a mixed positivity rate of 8.77%, respectively. The dominant pathogens were rotavirus (10.75%), norovirus (10.33%), enteropathogenic Escherichia coli (8.06%), Salmonella (6.36%), enteroaggregative Escherichia coli (5.52%), and Campylobacter (5.23%). Bacterial infections were predominant in summer and fall, and viral infections were predominant in winter and spring. The total positive rate decreased in 2020‒2023 compared with that of 2018‒2019 (χ2=5.753,P<0.05). Thirty-seven strains of Salmonella, 81 strains of diarrheagenic Escherichia coli,and 19 strains of Campylobacter were completed for the molecular typing analysis by PFGE, which were classified into 28, 80 and 18 banding types, respectively, with a wide range of banding similarity. Nineteen copies of norovirus GⅡ group gene sequences were analyzed and classified into 5 genotypes, which were mainly GⅡ.Pe-GⅡ.4 and GⅡ.P16-GⅡ.2 types. [Conclusion] Rotavirus, norovirus, diarrheagenic Escherichia coli, Salmonella, and Campylobacter are the dominant pathogens of infectious diarrhea in children in Putuo District of Shanghai. The pathogen spectrum shows a trend of seasonal epidemic characteristics, with a diversity of molecular characteristics of some pathogens. Surveillance and monitoring on molecular characteristics of the pathogens of infectious diarrhea in children should be strengthened in different seasons, so as to provide a laboratory basis for the prevention and control of infectious diarrhea.
Authors and Affiliations
GU Wenchao,ZHANG Huansheng,TANG Haifeng,YAN Hao,WU Tingting,GUI Yilin,LI Qiang,LI Yong,
Investigation and management of an anaphylactic shock case after inoculation with COVID-19 vaccine
Anaphylactic shock is a serious and rare adverse reactionï¼ which can be life-threatening if not treated in time. COVID-19 vaccine is a newly marketed vaccineï¼ and people pay high attention to its adverse reactions. T...
Risk prediction of low birth weight infants in Shanghai
ObjectiveTo investigate the risk factors of fertility behaviors with preterm birth and low birth weightï¼ and to develop a nomogram model to predict the occurrence of low birth weight.MethodsBirth registration informati...
Key issues and optimization strategies of emergency response to public health emergencies in the Yangtze River Delta region
ObjectiveTo identify the key issues in emergency response to public health emergencies in the Yangtze River Delta region, and to provide a basis for the formulation of relevant policies.MethodLiterature on emergency resp...
Visual analysis of social isolation studies on adolescents based on Web of Science
ObjectiveTo perform visual analysis of the literature in the field of adolescent social isolation in order to provide reference for research on social isolation of Chinese teenagers.MethodsLiterature related to adolescen...
A study of factors influencing the willingness of primary healthcare workers in Qinghai Province to engage in non-discriminatory behaviors towards HBV-infected patients
ObjectiveTo understand the current status of primary healthcare workersâ knowledge of hepatitis B and their willingness to engage in non-discriminatory behaviorsï¼ to explore the factors influencing their willingnessï...