Pathogenicity of entomopathogenic Entomophthora grylli on grasshoppers Diabolocatantops axillaris and Zonocerus variegatus causing leaf damage of shea tree seedlings
Journal Title: Journal of Agricultural and Crop Research - Year 2017, Vol 5, Issue 1
Abstract
The grasshoppers Diabolocatantops axillaris and Zonocerus variegatus were found feeding on the leaves of shea tree seedlings which caused damage that appears as rough patches on the surface. The objectives of this study were to provide healthy shea tree seedlings and control grasshoppers causing leaf damage using Entomophthora grylli as bioagent. Out of a total of 18 deaths termite mounds sand and forest soil samples that were examined, only 7 were found hosting E. grylli from Ihievbe Owan West with 26.5%, Ivbiaro Ebiaro Esako West with 9.2%, Eko-Igohebhe Esan Central with 8.7% and Umegbe Oredo with 2.8% respectively, all in Edo State. For the insect bait method, Orthoptera, D. axillaris and Z. variegatus, picked E. grylli from soil forest. Pathogenicity test revealed that injected E. grylli on grasshoppers with approximately 1 × 103 conidia consisting of mature resting spores (germinated and ungerminated) caused sluggish movement on grasshoppers on the eighth day. Grasshopper was found death as it clings to the aerial top of the container cover lid. Death of D. axillaris was observed on the eleventh day with mycelia growth on the abdomen. The results suggest that E. grylli could be an effective bioagent for the control of grasshoppers.
Authors and Affiliations
Eziashi E. I. , Chidi N. I.
Keywords
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