پاتوتايپينگ و بررسي شيوع اشريشياكلي‌هاي بيماري‌زاي روده‌اي در نمونه‌هاي آب ورودي به تصفيه‌خانه‌هاي شهر تهران
Journal Title: آب و فاضلاب - Year 2016, Vol 27, Issue 5
Abstract
Pathotyping of Escherichia coli Isolated from Inlets to Tehran Water Treatment Plants Water borne infections are of great importance for public health due to the role of water in the transmission of pathogens which cause gastrointestinal diseases. Traditional methods based on culture media commonly used for the identification of E. coli are not only time-consuming but fail to detect certain pathotypes of E. coli as well. To overcome these shortcomings, molecular methods were employed in the present study for the rapid and specific determination of E. coli pathotypes. For this purpose, 978 water samples were collected during the period from September 2012 to September 2013 and 106 E. coli strains were selected using multistep biochemical and molecular screening (tetraplex PCR) method. Virulogenes were determined by designing specific primers and developing efficient protocols. While it was shown that water has a great cabapility for transmiting pathogenic microorganisms, the results revealed that 10 strains contained the est, elt, and eaeA genes; five contained the bfpA gene; four contained the pCVD and ipaH genes; three contained the VT1 and VT2 genes; and finally one starin contained the cnf1 and cnf2 genes. It was also found that molecular methods based on our newly designed primers are sensitive, specific, and rapid protocols for pathotyping of Escherichia coli.
Authors and Affiliations
Maryam Asghari, Abbas Akhavan Sepahi, Seyyed Reza Hosseini Doost
Keywords
Related Articles
- EP ID EP179451
- DOI -
- Views 72
- Downloads 0
Preparation and Characterization of Magnetic Chitosan/-Carrageenan Nanocomposite and its Application for Removal of Eriochrome black-T from Aqueous Solutions تهيه و بررسي نانوکامپوزيت مغناطيسي کيتوسان/کاپا-کاراگينان و کاربرد آن در حذف رنگ اريوکروم بلاک تي
Abstract Due to the non-biodegradability of some dyes especially water soluble dyes, dye can pollute surface and groundwaters. Subsequently, discharging dye-contaminated wastewaters in the environment can endanger the h...
بررسي عوامل مؤثر بر قيمت پساب تخصيص داده شده به صنعت در استان اصفهان با استفاده از شبکه‌هاي عصبي مصنوعي
Using Artificial Neural Networks to Determine Significant Factors Affecting the Pricing of WPT Effluent for Industrial Uses in Isfahan The evidence indicates increasing trend of use of municipal wastewater treatment effl...
اصلاح سطح کربن فعال گرانوله با روش تصفيه نهايي با هدف نيترات‌زدايي از آب آشاميدني
Modification of Granular Activated Carbon with Post-Treatment to Enhance Nitrate Removal from Drinking Water The presence of nitrate in groundwater resources has instigated increasing concerns among both managers and use...
بررسي اثر اسيديته و اسيد هيوميک بر راندمان حذف نيترات از آب توسط نانوذرات آهن صفر
Effect of Humic Acid and Acidity on Nitrate Removal from Water Using Zero Valent Iron Nanoparticles (FeNps) Abstract Nitrate is a wide spread pollutant whose removal from aquesous solutions is a major goal of water tr...
بررسي الگوي مصرف آب خانگي با رويکرد بخش‌بندي مصرف‌کنندگان (مطالعه موردي: مصرف‌کنندگان آب خانگي شهرستان ايلام)
Investigating the Consumption Pattern of Domestic Water by Segmentation Approach for Water Consumers (Case Study: Domestic Water Consumers in Ilam City) The aim of this study is to identify domestic water consumption am...