Pattern And Sensitivity Test Against Bacteria In The Air At Ob/Gyn Operating Room Of Central Surgery Installation In Central General Hospital (Rsup) Haji Adam Malik Medan
Journal Title: IOSR Journal of Nursing and health Science - Year 2019, Vol 7, Issue 1
Abstract
Introduction and Objective: The incidence of nosocomial infections in Indonesia is in quite high number. The most common phenomenon is surgical wound infections, while it is getting worse by the increase of bacteria resistance due to the excessive and inappropriate use of antibiotics. This study aims to find pattern and sensitivity test against bacteria in the air at Ob/Gyn Operating Room, Central General Hospital (RSUP) H.Adam Malik. The research result expected to be use in order to find bacteria sensitivity against many antibiotics mostly used as prophylaxis of pre-operation and become reference whether the antibiotics are necessary and the type that should be used. Method: This study used descriptive observational design, while the research sample was contaminant bacteria taken from Ob/Gyn Operating Room in pre-operation for 2 weeks (10 working days) with "settled down plate" technique using Blood Agar medium. The research sample also taken from bacteria of patient’s wound swab that undergo surgery. Cultured bacteria were stained with Gram staining and sensitivity test conducted to the several types of antibiotics. The observation carried out in the Laboratory of Microbiology Faculty of Medicine, University of Sumatera Utara, Medan. Result: There is bacterial contamination in the air of operating room. The largest bacteria contaminant is Coagulase-negative Staphylococcus, Klebsiella pneumoniae, and Bacillus subtilis where the average growth rate during culture process is 12 colony-forming units (cfu)/plate (24 hours) and 19 cfu/ plate (48 hours). Sensitivity test shows that mostly used antibiotics are still sensitive, except Cefazoline that shows resistance. From 11 patients that conducted by wound swab, only 2 patients show bacteria growth. Conclusion: There is bacterial contamination found in the air of operating room so that the used antibiotics in pre and post surgery still needed.
Authors and Affiliations
Mario Markus Toar, Edy Ardiansyah, Mohammad Rhiza Tala, Sarma Nursani Lumbanraja, Henry Salim Sirega, Deri Edianto
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