Patterns of Gelatinase-B Expression in Leukemic Cell Lines
Journal Title: Iranian Journal of Blood and Cancer - Year 2010, Vol 2, Issue 3
Abstract
Background: Gelatinase-B named MMP-9 (matrix metalloproteinase-9), is a protease that degrades collagen type ІV and V of extracellular matrix. MMP-9 production is increased in various types of cancers including leukemia and has an essential role in tumor invasion, metastasis and angiogenesis. In this study, patterns of MMP-9 activity in a number of leukemic cells have been evaluated in-vitro. Materials and Methods: Human leukemic T cells (Jurkat and Molt-4) and monocyte (U937) were cultured in complete RPMI-1640 medium. Then the cells were seeded at a density of 10^(6) cells/ml and were incubated with different concentrations of PMA (1-25ng/ml) or PHA (1-10 µg/ml) for 24 hours. Afterwards, MMP-9 activity and MMP-9 protein level in cell-conditioned media were evaluated by gelatin zymography and ECL Western blotting, respectively. Results: PHA/ PMA significantly induced MMP-9 activity in Molt-4 and Jurkat cells after 24-hour incubation in a dose-dependent manner compared with untreated control cells. Moreover, PHA/ PMA extensively and dose dependently augmented MMP-9 activity in U937 cells after 24-hour incubation time compared with untreated control cells. Besides, PHA/ PMA increased MMP-9 level in U937 cells after 24-hour incubation time as was detected by western blotting compared with untreated control cells. Conclusion: According to the results of this study, human leukemic Jurkat, Molt-4, and U937 cells could exhibit MMP-9 activity with different extents. Among these cell lines, it seems that Molt-4 and U937 human leukemia cell lines, which greatly show MMP-9 activity after stimulation with PHA or PMA, may provide valuable tools for screening MMP enhancers or inhibitors and for assessment of regulatory mechanisms of MMP activity.
Authors and Affiliations
Fatemeh Hajighasemi, Sakineh Hajighasemi
Keywords
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