PCR-analysis of photoperiod sensitivity genes in bread wheat varieties from Bilatserkovska experimental breeding station
Journal Title: Вісник Українського товариства генетиків і селекціонерів - Year 2018, Vol 16, Issue 2
Abstract
The aim. Determination of alleles of the photoperiod sensitivity genes Ppd-1 in 16 winter wheat varieties of the Bilatserkovska Experimental Breeding Station and identification of the haplotypes of Ppd-D1 gene according to the accepted classification. Methods. DNA isolation, allele-specific and nested PCR, electrophoresis in agarose and polyacrylamide gels, determination of the significant differences in the time of heading. Results. Plants of the varieties Vodohrai bilotserkivs’kiy; Bilotserkivs’ka napivkarlykova, Olesia, Perlyna lisostepu, Elehiia, Yasochka, Lybid’, Tsarivna, Lisova pisnia, Romantyka, Vidrada, Schedra nyva, Charodiika bilotsekivs’ka, Russa, Driada 1 have the genotype PpdA1b Ppd-B1b Ppd-D1a and belong to the VII haplotype of the Ppd-D1 gene. The genotype of the variety Legenda bilotsekivs’ka is characterized by the presence of the alleles Ppd-A1b Ppd-B1b Ppd-D1b and belongs to the IV haplotype of the Ppd-D1 gene. The significant differences (P = 0.01) in the time of heading was detected only between the varieties Russa and Legenda bilotsekivs’ka and was 12.4 days. Conclusions. Most BEBS varieties are characterized by genotype — PpdA1b Ppd-B1b Ppd-D1a, in which the allele Ppd-D1a determines insensitivity to the photoperiod and leads to early heading. Only variety Legenda bilotsekivs’ka is the carrier of the recessive allele of Ppd-D1 gene, belongs to the IV haplotype, has the latest time of heading. Keywords: Ppd-A1, Ppd-B1, Ppd-D1 genes, winter wheat, PCR analysis, photoperiod sensitivity.
Authors and Affiliations
V. M. Filimonov, A. A. Bakuma, G. A. Chebotar, L. A. Burdenyuk-Tarasevich, S. V. Chebotar
Keywords
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- EP ID EP613141
- DOI 10.7124/visnyk.utgis.16.2.1060
- Views 75
- Downloads 0
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