PCR and Culture Analysis of Enterococcus Faecalis from Retreatment Cases in Indian Population
Journal Title: Saudi Journal of Oral and Dental Research - Year 2018, Vol 3, Issue 3
Abstract
Abstract: Enterococcus faecalis has long been mentioned in increased frequency with regard to teeth with post-treatment disease (PTD). The prominence of E. faecalis in root-filled teeth with apical periodontitis has made it a focus of attention as an etiological factor of PTD. To evaluate and compare bacterial culture and species-specific polymerase chain reaction for the detection of enterococcus faecalis in retreatment cases with apical periodontitis in rural Indian population. A total of 80 samples were obtained from 40 patients, divided into 2 groups and analyzed by the two methods. The samples were grown under anaerobic conditions during culture and the determination of the microbial species was based on biochemical tests. DNA was extracted and analyzed for PCR using species-specific primer. The association of microbiological findings with clinical features was investigated. Enterococcus faecalis was isolated from 46.5% of the selected teeth. Culture and PCR detected the test species in 25% and 67.5% of the samples. PCR was significantly more effective than culture in detecting this bacterial species. E.faecalis was clearly proven as the causative factor of apical periodontitis. Therefore, combining molecular and culture technique is probably the best approach available to provide comprehensive and a panoptic information about the microflora associated with endodontic infections. Keywords: Enterococcus faecalis, apical periodontitis, polymerase chain reaction, DNA, Culture techniques.
Authors and Affiliations
Nithya Pushpanathan, Kalpesh Revankar, Manish Kumar, Monika Sharma
Keywords
Related Articles
- EP ID EP400289
- DOI -
- Views 85
- Downloads 0
Impacted Mandibular Third Molar in Ramus: A Rare Presentation
Abstract: Mandibular third molars were found to be the common impacted tooth, but the ectopic eruption of the third molar is uncommon. The etiology and other information of these teeth were not completely clarified. The...
Endodontic management of foreign body in root canal of 10 year child: a case report
Abstract: Over the course of time, dentistry has evolved from a primitive form of dental care to modern day use of preventive treatment modalities. In today’s modern dentistry with the availability of various new obturat...
Management of Endodontic Complication by Intentional Replantation
Abstract: Complete cleaning and shaping of the root canal system is essential for success of endodontic treatment. Accurate measurement of working length and 3D obturation till the apical constriction is necessary for en...
Clinical anxiety among junior dental students: Trainers and students’ perspective
Abstract: The aim of this study was to determine provoking factors of clinical anxiety among dental students from trainers and students’ perspective. A cross-sectional study was conducted among junior dental students of...
CAD-CAM technology and immediate loading for advanced dental rehabilitation.
Abstract: The world of implant ology is continuously expanding. The application of computer-aided design and computer-aided manufacturing (CAD/CAM) to advanced dental rehabilitation programs is one of the most interestin...