PCR and Culture Analysis of Enterococcus Faecalis from Retreatment Cases in Indian Population
Journal Title: Saudi Journal of Oral and Dental Research - Year 2018, Vol 3, Issue 3
Abstract
Abstract: Enterococcus faecalis has long been mentioned in increased frequency with regard to teeth with post-treatment disease (PTD). The prominence of E. faecalis in root-filled teeth with apical periodontitis has made it a focus of attention as an etiological factor of PTD. To evaluate and compare bacterial culture and species-specific polymerase chain reaction for the detection of enterococcus faecalis in retreatment cases with apical periodontitis in rural Indian population. A total of 80 samples were obtained from 40 patients, divided into 2 groups and analyzed by the two methods. The samples were grown under anaerobic conditions during culture and the determination of the microbial species was based on biochemical tests. DNA was extracted and analyzed for PCR using species-specific primer. The association of microbiological findings with clinical features was investigated. Enterococcus faecalis was isolated from 46.5% of the selected teeth. Culture and PCR detected the test species in 25% and 67.5% of the samples. PCR was significantly more effective than culture in detecting this bacterial species. E.faecalis was clearly proven as the causative factor of apical periodontitis. Therefore, combining molecular and culture technique is probably the best approach available to provide comprehensive and a panoptic information about the microflora associated with endodontic infections. Keywords: Enterococcus faecalis, apical periodontitis, polymerase chain reaction, DNA, Culture techniques.
Authors and Affiliations
Nithya Pushpanathan, Kalpesh Revankar, Manish Kumar, Monika Sharma
Keywords
Related Articles
- EP ID EP400289
- DOI -
- Views 84
- Downloads 0
Diastema and Frenum – An Insight
Abstract: The midline diastema is a space (or gap) between the maxillary central incisors . The space can be a normal growth characteristic during the primary and mixed dentition and generally is closed by the time the m...
A Clinical and Histomorphometric Evaluation of Socket Preservation Using Resorbable Collagen Membrane and an Alloplast: A Comparative Interventional Study
Abstract: There are various grafting materials used for socket preservation of the alveolar ridge following tooth extraction. The purpose of this study was to evaluate clinically and histomorphometrically healing after t...
OHRQoL: Oral Health Related Quality of Life : Complete Dentures
Abstract: When assessing the outcomes of dental treatment, it is important to consider the clinicians as well as the patients’ point of view. The dentist must attempt to restore, most often elderly patient with mental/ph...
Management of a Large Periapical Cyst – Report of a Case and Review of Literature
Abstract: Radicular cysts are one of the most common pathology of oral cavity that oral and maxillofacial surgeons come across in daily practice. It has different treatment options and an operating surgeon should be awar...
Conservative Treatment Approach for Management of Fractured Maxillary Canine with Surgical Crown Lengthening and Post Core Restoration
Abstract:Perpetual preservation of what is remaining is more important than meticulous reconstruction of what is lost. Preservation and restoration of badly carious or broken tooth possess problems due insufficient remai...