PCR detection of white spot syndrome virus (WSSV) from farmed Pacific white shrimp (Litopenaeus vannamei) in selected sites of the Philippine
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2011, Vol 4, Issue 4
Abstract
Great losses caused by white spot syndrome virus (WSSV) in shrimp culture have been attributed to poor screening procedures in farms and the lack of sufficient access to specific pathogen free brood stock. Thus, early detection of the virus is considered the best option for shrimp farmers. The study, thus, assessed viral incidence in the Philippines and partially sequenced and characterized the Philippine WSSV isolate with regards to other isolates in GenBank. Developed primers for PCR can detect target genes from 0.4 pg of DNA extract from shrimp samples. PCR detection revealed that 6.67 % (1/15) of market samples from Zambales are infected with WSSV. Shrimp samples from a local shop and a public market in General Santos City showed 46.67% (7/15) and 20% (3/15) WSSV-positive samples respectively. Shrimp sources from Capiz and Batangas, however, showed negative detection for WSV. No significant difference in the number of infected samples from the sampling sites was found. Combined detections reveal that the Philippines has a low infection rate of 14.67%. The study has partially sequenced and characterized Philippine isolate. During the sampling period, most shrimps in General Santos City were WSSV-positive by PCR detection.
Authors and Affiliations
Benedict Maralit, Christopher Caipang, Mudjekeewis Santos, Mary Maningas
Keywords
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