Peptidome profiling of human serum of uveal melanoma patients based on magnetic bead fractionation and mass spectrometry
Journal Title: International Journal of Ophthalmology - Year 2017, Vol 10, Issue 6
Abstract
"AIM: To find new biomarkers for uveal melanoma (UM) by analyzing the serum peptidome profile. METHODS: Proteomic spectra in patients with UM before and after operation were analyzed and compared with those of healthy controls. Magnetic affinity beads were used to capture serum peptides and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer were used to compile serum peptide profiles. RESULTS: A panel of 49 peptides were differentially expressed between UM patients and controls, of which 33 peptides were of higher intensities in patient group and 16 peptides were of higher intensities in control group. Based on combined use of these potential markers, peptides with mean molecular masses of 1467 and 9289.0 Da provide high sensitivity (83.3%), specificity (100%) and accuracy rate (93.0%) together to differentiate melanoma patients from healthy controls. At the time point of 6mo postoperatively, the levels of many peptides differentially expressed before surgery showed no more statistical difference between the patients and the control group. Fibrinogen α-chain precursors were identified as potential UM markers. CONCLUSION: We have shown that a convenient and fast proteomic technique, affinity bead separation and MALDI-TOF analysis combined with bioinformatic software, facilitates the identification of novel biomarkers for UM."
Authors and Affiliations
Li-Ming Tao
Keywords
Related Articles
- EP ID EP602850
- DOI 10.18240/ijo.2017.06.17
- Views 54
- Downloads 0
Contralateral eye study of refractive, topographic and aberrometric outcomes after femtosecond assisted MyoRing implantation and DALK for management of keratoconus
"AIM: To evaluate the efficacy of femtosecond laser assisted MyoRing intrastromal corneal implant and deep anterior lamellar keratoplasty (DALK) for management of moderate to advanced keratoconus regarding the degree of...
The 100 most influential papers about cataract surgery: a bibliometric analysis
"AIM: To identify the 100 most cited papers in cataract surgery, we performed a comprehensive bibliometric analysis basing on the literature search on the Thomson Reuters Web of Knowledge. METHODS: The number of citation...
The role of elastic fibers in pathogenesis of conjunctivochalasis
The PubMed, MEDLINE databases and China National Knowledge Infrastructure (CNKI) were searched for information regarding the etiology and pathogenesis of conjunctivochalasis (CCh) and the synthesis and degradation of ela...
Peripheral ulcerative keratitis and otitis media as initial symptoms of Wegener’s granulomatosis: a case report
Letter to the editor
Effect of infusion pressure during cataract surgery on ganglion cells measured using isolated-check visual evoked potential
"AIM: To detect the relationship between infusion pressure and postoperative ganglion cells function. METHODS: This prospective observational cohort study included sixty-one eyes that underwent uncomplicated cataract sur...