Pharmacokinetic study of dl-tetrahydropalmatine patches by UPLC-MS/MS in rabbits

Apply

Pharmacokinetic study of dl-tetrahydropalmatine patches by UPLC-MS/MS in rabbits

Journal Title: Traditional Medicine Research - Year 2016, Vol 1, Issue 4

Abstract

The paper established the determination method of the plasma concentration of dl-tetrahydropalmatine patch via rabbit skin. When treating a blood sample, the study mainly researched chloroform and ethyl acetate as the purified extraction solvent. By comparison, It’s found that chloroform dissolution rate and solubility to dl-tetrahydropalmatine were higher than ethyl acetate, and it could be extracted completely within a few seconds. Whereas extracted dl-tetrahydropalmatine using chloroform , the residual degree in the vessel wall was far less than the ethyl acetate. Through the average recovery test, it was found that extraction and purification using chloroform could fully meet the test requirements. For the dl-tetrahydropalmatine patch, it can be found that the water content was a great impact factor on the transdermal efficiency, so it must be controlled more than 33% in the experiment, and primarily with the stratum corneum (SC) water-related. Appropriately increasing the humidity can affect skin SC hydration and then the skin SC cells loosely arranged, meaning enhancing the transdermal effect. According to the authentication and related data, it was indicated that using UPLC-MS/MS to determine plasma concentrations of the dl-tetrahydropalmatine patch via rabbit skin is simple, convenient and fast. Objective: To establish the measurement method of the plasma concentration and inspect the pharmacokinetic of the dl-tetrahydropalmatine patches. Method: Tandem mass spectrometric detection (UPLC-MS/MS) was employed for the detection of the plasma concentration. Results: We established the mass spectrometry conditions of dl-tetrahydropalmatine, all of which could meet the requirements of analysis of biological samples. The distribution law of dl-tetrahydropalmatine patches accorded with the single compartment model; Tmax=5.0h, Cmax=153.12ng/ml, MRT=10.0h, AUC0-inf=2.10 ug/ml·h, AUMC=21.15ug/ml·h2. Conclusion: The UPLC-MS\MS method was useful in the pharmacokinetic study of dl-tetrahydropalmatine patches. It was simple, rapid, accurate and reliable and had high sensitivity and good reproducibility. Consequently, the method could be available for quantitative analysis and pharmacokinetic studies of dl-tetrahydropalmatine patches.

Authors and Affiliations

Xie Wei-Jie, Zhang Yong-Ping, Xu Jian

Keywords

UPLC-MS\MS|Dl-tetrahydropalmatine patch|Plasma concentration|Pharmacokinetics

A multi-center randomized controlled clinical trial of three-step acupuncture and cupping therapy for cervicogenic headache

Objective: To assess the clinical efficacy and safety of three-step acupuncture and cupping therapy for cervicogenic headache (CEH). Methods: A randomized, single-blind, multi-center, parallel controlled clinical trial w...

Molecular mechanism prediction analysis of compound Kushen injection in the treatment of COVID-19 based on network pharmacology and molecular docking

Background: As one of the eight effective traditional Chinese medicines for the treatment of atypical pneumonia, compound Kushen injection (CKI) played an important role in combating pneumonia caused by severe acute resp...

EP ID EP45282
DOI 10.12032/TMR201604028
Views 297
Downloads 0