Pharmacokinetic study of subcutaneous administration of melatonin in broilers with thermosensitive in situ gel

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Pharmacokinetic study of subcutaneous administration of melatonin in broilers with thermosensitive in situ gel

Journal Title: Journal of Henan Agricultural University - Year 2023, Vol 57, Issue 3

Abstract

[Objective] To compare the pharmacokinetic characteristics of melatonin thermosensitive in situ gel administered subcutaneously in broilers with melatonin solution as the control, so as to provide evidence and guidance for the clinical use of melatonin thermosensitive in situ gel. [Method] Broilers were given 3 mg·kg-1 melatonin by a single subcutaneous injection of thermosensitive in situ gel and solution. Blood samples were collected at 0.08, 0.16, 0.25, 0.50, 0.75, 1, 1.50, 2, 4, 6, 8, 9, 24, 48, 60, 72, 84, 96, 108, and 120 h after drug administration, respectively. The melatonin quality concentrations of the two dosage forms in broiler plasma were measured and compared by high performance liquid chromatography. And the pharmacokinetic model of thermosensitive in situ gel was established by analyzing the pharmacokinetic parameters of two dosage forms of melatonin in broiler blood. [Result] The average peak mass concentration (Cmax)of melatonin in plasma of broilers after a single dose of subcutaneous injection in the thermosensitive in situ gel group and the melatonin solution group was (561.66±0.82) and (890.22±0.20) mg·L-1, respectively, and the difference was significant between the two groups (P< 0.05). The peak time tmax was 72.00 and 0.50 h, the half-life T1/2 was 22.49 and 0.58 h, the average retention time MRT(0-∞) was (77.320±2.590) and (1.108±0.002) h·mg·L-1, respectively. The AUC(0-∞) of the drug time curve was (39 068.75±1 514.00) and (890.70±1.02) h·mg·L-1, respectively, and the difference between the groups was significant(P< 0.01). The relative bioavailability (F) of melatonin temperature-sensitive in situ gel was (43.89±14.84)%. [Conclusion] At the same dose, the absorption and elimination rates of temperature-sensitive in situ gel were slower than those of the solution, indicating that temperature-sensitive in situ gel could improve the absorption and distribution of melatonin.

Authors and Affiliations

Yaxing ZHOU, Yueying WANG, Furong NIE, Taiyu LIU

Keywords

Multidimensional performance evaluation and obstacle factor diagnosis of farmland shareholding cooperatives in traditional rural areas

[Objective] The establishment and development of farmland shareholding cooperatives is the product of the reform and innovation of agricultural land transfer system. The multi-dimensional performance and obstacle factors...

EP ID EP769213
DOI 10.16445/j.cnki.1000-2340.20221212.002
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