PHARMACOLOGICAL EVALUATION AND DETECTION OF ANACARDIC ACID IN CALLUS CULTURE AND VARIOUS PLANT PARTS OF ANACARDIUM OCCIDENTALE L.
Journal Title: International Journal of Pharmaceutical Sciences and Drug Research - Year 2015, Vol 7, Issue 3
Abstract
Anacardium occidentale L. is much known for its commercially valuable kernals and also bioactive compounds like polyphenols which serve as natural antioxidant having an ability to quench cytotoxic free radicals that may prevent many diseases. The present study was carried out to evaluate the presence of anacardic acid, total phenolic compound, antioxidant activity and antibacterial activity in methanolic extract of callus and various plant parts such as flower, young leaves, shoot and cotyledon. Total phenolic contents were measured by the Folin-Ciocalteau method using gallic acid as standard compound. The maximum phenolic content was found in the methanol extract of flower (260.8 ± 5.543 mg/g) whereas the methanol extract of cotyledon (16.2 ± 3.284 mg/g) showed the lowest total phenolic content. The action of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) on radical scavenging effect of the extracts was determined spectrophotometrically using ascorbic acid as standard compound. All extracts exhibited a DPPH radical scavenging activity and among the extracts, A. occidentale flower demonstrated greater antioxidant potential with a low IC50 (206.312 ± 0.38µg/ml) in comparison with those of the other extracts. Total phenolic contents showed positive correlations with the DPPH radical scavenging activity (r = 0.979; p<0.01) and negative correlations with IC50 (r = -0.984; p<0.01). The antibacterial capabilities of various extracts were also investigated against two common human pathogens of clinical importance, Escherichia coli and Staphylococcus aureus. Presence of anacardic acid in various extracts was also estimated by using HPTLC method. Pre-coated aluminum silica gel plate 60F254 was used as stationary phase and chloroform: ethyl acetate (9:1) was used as mobile phase.
Authors and Affiliations
Sija. S. L. , Potty. V. P. , Santhoshlal. P. S.
Keywords
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