PHASE DEPENDENT DISCREPANCY IN MURINE VAGINAL MICRO-ENVIRONMENT: A CORRELATIVE ANALYSIS OF pH, GLYCOGEN AND SERUM ESTROGEN UPON EXPOSURE TO LAPATINIB DITOSYLATE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 5
Abstract
Objective: This present investigation was designed to correlate the Hydrogen-ion concentration of vaginal fluid with glycogen content and serum estrogen level during different phases of mice reproductive cycle. These parameters were compared with the control and treated animals to set off this factor in the field of reproductive toxicological studies.Methods: Female Swiss Albino mice [6-8 w] with regular estrous cyclicity were separated out into two groups as control [only HPMC] and treated [lapatinib in HPMC] animals. The treatment was carried out consecutively for 21 d via oral gavage at a dose of 20 mg/kg/d. Estrous cycle, pH and glycogen content of vaginal fluid was monitored on daily basis. At the end of the experiment period, serum estrogen level was quantified by ELISA method.Results: Significant changes were observed in diestrus index, estrogen, and glycogen content with respect to their reproductive phases. In particular, the higher variations, seen within and between groups in estrous phase, suggest the maximum inhibitory potential exhibited by the drug used, was due to the elevated expression of its receptors [EGFR] at the time of oocyte maturation and ovulation.Conclusion: Even, a change by one pH unit may illustrate the difference between healthy and heavily infected vaginal flora. Although, the pH value of vaginal secretions is not routinely used and compared with other parameters in the toxicological studies. This present study has become the trend set for considering pH as a valuable tool in in vivo studies.Keywords: Estrous cycle, Vaginal pH, Phenol red, Glycogen, Estrogen, Correlation analysis
Authors and Affiliations
Mahadevan Ganesan, Balamuthu Kadalmani
Keywords
Related Articles
- EP ID EP577672
- DOI -
- Views 83
- Downloads 0
BANANA PEEL OXALATE OXIDASE-DETECTION, PURIFICATION, CHARACTERIZATION AND PHYSIOLOGICAL ROLE
Objective: Enzymes like oxalate oxidase (EC 1.2.3.4) and superoxide dismutase (EC 1.15.1.1) from germin family are known to generate active oxygen species. In the mammalian system, excess accumulations of oxalate causes...
STUDY ON CAUSE–EFFECT RELATIONS AND OPTIMIZATION OF EXEMESTANE-LOADED NANOSTRUCTURED LIPID CARRIERS
Objective: Exemestane is an anti-breast cancer drug, possesses low water solubility and low permeability. This work aimed at the cause-effect relations and optimization of exemestane-loaded nanostructured lipid carriers...
DISCRIMINANT ANALYSIS OF SHODHANA (PROCESSING) ON BALIOSPERMUM MONTANUM MUELL (DANTI) ROOT SAMPLES BASED ON NEAR INFRARED SPECTROSCOPY AND MULTIVARIATE CHEMOMETRIC TECHNIQUE
Objective: To establish a noticeable and a justifiable identification system to assess the impact of shodhana (processing) on various levels of Baliospermum montanum (Danti) root samples obtained through shodhana (proces...
Validation of two spectrophotometric methods for fluoxetine quantification
Objective: This paper describes the development and validation of two UV-Visible spectrophotometric analysis methods, using absolute ethanol (method A) and HCl 0.1 M (method B) as solvents, to quantify fluoxetine (FLX) i...
PREPARATION OF RICE STRAW ACTIVATED CHARCOAL BY 2- STEP H3PO4 ACTIVATION
Objective: To determine optimal conditions required for activated charcoal production from rice straw with high quality for medical and pharmaceutical applications using chemical activation process.Methods: Effects of di...