Phenotypic and genotypic characterization of oxidase positive Gram negative bacilli isolated from broiler chickens.
Journal Title: UNKNOWN - Year 2018, Vol 25, Issue 1
Abstract
The current work aimed to study the phenotypic and genotypic characters of oxidase positive Gram negative bacterial pathogens recovered from different pathological lesions in broiler chickens. Samples were taken from 200 Hubbard and Ross broiler chickens of different ages (3-5weeks), from different farms in Beni-Suef and El-Fayoum Governorates during the period from January 2016 to April 2016. Bacteriological examination showed that Gram negative bacteria were 165 (82.5%) of isolates of which 60 isolates (30%) were oxidase negative while 105 isolates (52.5%) were oxidase positive including 43 Pseudomonas aeruginosa, 35 Aeromonas hydrophila, 12 Pasteurella gallicida, 10 Plesiomonas shigelloides, and 5 Vibrio vulnificus with incidences of 21.5%, 17.5%, 6% 5%, and 2.5%, respectively. The in-vitro sensitivity tests were applied on a total of 59 isolates; 20 P. aeruginosa, 19 A. hydrophila, 10 P. gallicida, 5 P. shigelloides and 5 V. vulnificus against 13 different antimicrobial agents and multidrug resistant isolates were detected. Multiplex-PCR was applied on 15 different MDR isolates. The results of PCR revealed that blaTEM, CIT and FOX genes were the most prevalent where they were found in 8 isolates (53.3%) followed by blaSHV which was found only in 5 isolates (33.3%).
Authors and Affiliations
Walid H. Hassan1, Ahmed H. Abed1, Soad Abd-El Aziz Abd-Alwanis 2, Marwa Ahmed Yahia AlSayed 3 1 Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt 2 Animal Health Research Institute Dokki, Egypt 3 Animal Health Research Institute Beni-Suef, Egypt
Keywords
Related Articles
- EP ID EP102979
- DOI 10.21608/jvmr.2018.43300
- Views 162
- Downloads 0
Phenotypic and genotypic characterization of methicillin resistant Staphylococcus aureus.
Food contaminated with multiple antibiotic-resistant S.aureus can be a major threat to the public health. The purpose of this study was to isolate S.aureus from different food sources, determine their antimicrobial sus...
Field evaluation of inactivated Corynebacterium ovis vaccine in sheep prepared from a local isolate
Corynebacterium pseudotuberculosis vaccine was prepared from a local field isolate. Vaccination of sheep with 50g PLD toxoid and 10 mg bacterin adjuvanted with Montanide oil improved the levels of immune responses of...
Monitoring the hygienic quality of underground water in different localities in Egypt and Libya
The present study was carried out in six provinces in both Egypt and Libya throughout the period from January to October 2014. These areas were (Beni-Suef, Ismailia, and Matrouh district) in Egypt and (Tripoli, Zliten,...
Characterization of E. coli and Salmonella spp. isolates associated with omphalitis in baby chicks
Omphalitis is a major cause of increased first week-chick mortality. Omphalitis, navel-yolk sac infection, is a hatchery-born disease, and also known as ‘mushy chick disease’ or ‘navel ill’. It is a common disease of c...
Physicochemical and bacteriological status of retail-marketed shrimps and crabs in Beni-Suef, Egypt
A total of a hundred and twenty samples of crustaceans, 60 samples of shrimp (30 peeled & 30 unpeeled shrimps) and 60 samples of local chilled crab were collected from Beni-Suef markets. Collected samples were evaluate...