Phenotypic characterization of canine Malassezia spp., isolates
Journal Title: Revista MVZ Cordoba - Year 2016, Vol 21, Issue 3
Abstract
Objective. To characterize and identify yeasts of the genus Malassezia by phenotypic features. Materials and methods. First, the macroscopic and microscopic morphological characteristics were described. In addition we performed biochemical and physiological assays as Tweens and Cremophor, including more. Results. Our results evidenced of 105 isolates obtained from dogs diagnosed with external otitis, it was possible to identify two distinct species from 46 isolates within the Malassezia genus: 36.19% (n=38) were identified as M. pachydermatis and 7.62% (n=8) as M. furfur. According to phenotypic patterns the remaining 56.19% (n=59) were reported as Malassezia spp., possibly corresponding to M. furfur and/or M. pachydermatis. Conclusions. Results emphasize the necessity to characterize according to species. It is not feasible to define Malassezia by species based on morphological, biochemical, and physiological findings. Therefore, molecular genotyping should be performed to identify markers allowing a more precise isolate identification. This would broaden our epidemiological knowledge regarding different species involved in canine otitis pathologies.
Authors and Affiliations
Angélica Hurtado-Suárez, Bact| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología. Unidad de Investigaciones Agropecuarias (UNIDIA). Línea de Epidemiología y Salud Animal. Carrera 7a No. 42- 83. Ed 52 Of. 608. Bogotá- Colombia., Adriana Pulido-Villamarín, M. Sc *| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología. Unidad de Investigaciones Agropecuarias (UNIDIA). Línea de Epidemiología y Salud Animal. Carrera 7a No. 42- 83. Ed 52 Of. 608. Bogotá- Colombia.Correspondence: adriana.pulido@javeriana.edu.co, Melva Linares-Linares, M. Sc| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología. Unidad de Investigaciones Agropecuarias (UNIDIA). Línea de Epidemiología y Salud Animal. Carrera 7a No. 42- 83. Ed 52 Of. 608. Bogotá- Colombia., Leidy Suárez-Fernández, Bact| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología. Unidad de Investigaciones Agropecuarias (UNIDIA). Línea de Epidemiología y Salud Animal. Carrera 7a No. 42- 83. Ed 52 Of. 608. Bogotá- Colombia., Rubiela Castañeda-Salazar, M. Sc| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología. Unidad de Investigaciones Agropecuarias (UNIDIA). Línea de Epidemiología y Salud Animal. Carrera 7a No. 42- 83. Ed 52 Of. 608. Bogotá- Colombia., María Rodríguez-Bocanegra, Ph. D| Pontificia Universidad Javeriana. Facultad de Ciencias. Departamento de Microbiología. Unidad de Investigaciones Agropecuarias (UNIDIA). Línea de Epidemiología y Salud Animal. Carrera 7a No. 42- 83. Ed 52 Of. 608. Bogotá- Colombia.
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