Phenotypic detection of ESBL among E.coli and Klebiella pneumoniae by modified CLSI guidelines
Journal Title: IP International Journal of Medical Microbiology and Tropical Diseases - Year 2018, Vol 4, Issue 3
Abstract
Introduction Escherichia coli and Klebsiella pneumoniae cause a wide range of infections Multidrugresistance strains carrying resistance genes have become a growing problem worldwide The ESBLs have emerged distinctly especially in Escherichia coli and Klebsiella pneumoniaeMaterial and Methods 250 non repetitive isolates of E coli and K pneumoniae were isolated from different clinical samples pus urine sputum blood for the study Each isolate was tested for production of ESBL by CLSI recommended PCT and compared with Modified CLSI Method for ESBL detectionResults 133 E coli and 117 K pneumoniae were isolated where 652 of ESBL were detected With modified CLSI method overall rate of ESBL positivity increased from 652 to 744of E coli and 743 of K pneumoniae isolates were ESBL producers by modified CSLI MethodConclusion All the clinical samples growing gram negative bacteria should be tested for ESBL production Considering the grave scenario of antibiotic resistance in our country it is high time that all clinical laboratories start detecting these enzymes routinely and accuratelyKeywords Escherichia coli Klebsiella pneumoniae ESBL Modified CLSI guidelines
Authors and Affiliations
Sumangala B, Akshantha B Sangannavar, Sahana Shetty N. S
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