Phenotypic Evaluation of Cassava (Manihot esculenta Crantz) Genotypes to Cassava Mosaic Virus by Mechanical Methods of Transmission
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2017, Vol 16, Issue 3
Abstract
Aims: Cassava (Manihot esculenta Crantz), subsistence crop in sub-Saharan Africa was threatened by cassava mosaic virus that caused a devastated disease. This study aims to test thermotherapy as sanitation method in mechanical transmission study of cassava mosaic virus. Methodology: Cuttings of different cultivars were treated by heating during two hours and twenty four hours at 50°C before potting. Four mechanical techniques of virus transmission were used. Contact technique consisting to put an infected plant with a healthy plant together by one junction point and the graft technique involved the grafting of axillary buds of diseased plants to healthy plants. Latex technique consisted of passing the latex from the infected plants on the injured healthy plants several times and sap technique involved the injection of inoculum prepared from the infected leaves to the healthy plants. Results: Four cultivars had a sprouted rate superior to 80% by two hours of heating and less than 50% by 24 hours of heating. The bands related to ACMV or EACMV were not observed in the samples of different cultivars. Seventy five per cent (75%) of plants inoculated by contact technique had a perfect adhesion with 65% of plants expressing the mosaic symptoms. Six plants out of fourteen (6/14) of the plants of Hombete inoculated with latex expressed the disease symptoms and three plants out of ten (3/10) of the plants of Ornania expressed the disease symptoms. Hundred per cent (100%) of the plants of Djadjakor inoculated by grafting expressed the disease symptoms. Any plants of Atinwewe and Adjatidaho inoculated by Sap technique did not express the disease symptoms. Conclusion: These results suggest that heat is effective for virus elimination and grafting constitutes the mechanical transmission technique which can be used to screen cassava germplasm for virus resistance.
Authors and Affiliations
Jerome Anani Houngue, Gilles Habib Todjro Cacaï, Justin S. Pita, Martine Zandjanakou -Tachin, Edwige Fougnigué Yeo, Corneille Ahanhanzo
Keywords
Related Articles
- EP ID EP318163
- DOI 10.9734/JABB/2017/38149
- Views 75
- Downloads 0
Total Phenols, Antioxidant Capacity and Antibacterial Activity of Manuka Honey Extract
Aims: To evaluate total phenols content (TPC), antioxidant capacity (TAC) and antibacterial activity of Manuka honey extract (MHE) and to compare such properties with those for unfractionated Manuka honey. Study Design...
Bio-ethanol Production as Bio-solvent (Antifermenter), Antiseptic and Bio-fuel from Date Fruit Waste and its Suitable Properties Identification
Significance of the Study: Biomass derived bio-ethanol is biodegradable, nontoxic and suitable substitute for fossil fuels. It can be used as bio-solvent and antiseptic in the pharmaceutical industry and can also be used...
Isolation and Characterization of Bioflocculant-Producing Bacteria from Wastewater at Jimeta, Adamawa State
Aim: The aim of this study is to isolate, identify and screen for bacteria capable of producing bioflocculant from three wastewater disposal sites; Jimeta Abattoir, Jimeta Modern Market and the Gwari Market waste disposa...
Nutrient and Mineral Content of Oyster Mushroom (Pleurotus florida) Grown on Selected Lignocellulosic Substrates
This study was carried out to determine the proximate and mineral content of Pleurotus florida mushroom in order to assess its nutritional value and to determine its yield and biological efficiency on the substrates. Ple...
Manuka Honey-induced Cytotoxicity against MCF7 Breast Cancer Cells is Correlated to Total Phenol Content and Antioxidant Power
Aims: To investigate the relations between total polyphenols content, antioxidant power and Manuka honey cytotoxicity towards MCF-7 cells. Study Design: In vitro study. Place and Duration of Study: Department of Chemistr...