Phenotyping and Molecular Characterization of Extended-Spectrum Beta-Lactamases among Clinical Isolates of Gram-Negative Bacilli in Arar Tertiary Care Hospital, Saudi Arabia
Journal Title: Journal of Communicable Diseases - Year 2018, Vol 50, Issue 1
Abstract
Objectives: The emergence of extended-spectrum β-lactamases (ESBLs) in drug-resistant Gram-negative bacilli isolates in critically ill patients is posing a serious threat. Lack of data on the prevalence of drugresistant Gram-negative bacilli with respect to enzymes responsible for drug resistance in the tertiary care hospital at Arar necessitated this study. Methods: All the Gram-negative bacilli isolated from clinical specimens were subjected to antibiotic susceptibility testing. Drug-resistant organisms were screened for the presence of ESBLs, and confirmed by phenotypic methods. Molecular characterizations of these organisms were performed by conventional PCR to identify the ESBLs genes. Results: A total of 3711 specimens were processed over a period of one year and 428 Gram-negative bacilli (GNB) were isolated. Out of 428 GNB, 91 (21.2%) were ESBL producers which include Escherichia coli (46), Klebsiella pneumoniae (33), Proteus mirabilis (11), and Klebsiella oxytoca(1). The minimum inhibitory concentration (MIC) for ceftazidime and ceftazidime plus clavulanic acid for all the ESBL (91) isolates were >16 μg/mL and <0.25 μg/mL whereas for cefotaxime and cefotaxime plus clavulanic acid the MIC was >32 μg/mL and <0.25 to 0.5 μg/mL respectively. CTX gene 44(48.3%), was predominant among all ESBL producers; next to it was SHV 15(16.5%) and TEM 2(2.2%), and 30(32.9%) isolates had all the three genes (CTX, SHV, and TEM). Conclusion: The findings in our study revealed the higher prevalence of ESBL in the community. Surveillance of antibiotic resistance with respect to ESBLs will provide a platform for framing antibiotic policy.
Authors and Affiliations
Dr. Jayakumar Subramaniam,
Keywords
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- EP ID EP284967
- DOI 10.24321/0019.5138.201805
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