Phylogenetic analysis of Dengue Virus Serotype 1 Isolated from Clinically Suspected Pediatric Patients in Chennai, Tamilnadu.
Journal Title: International Journal of Advanced Biotechnology and Research. - Year 2016, Vol 7, Issue 1
Abstract
ABSTRACT The evolution of Dengue virus has a major impact on their virulence in humans and on the epidemiology of dengue disease around the world. In order to perform disease surveillance and to understand DENV evolution, the molecular studies with Bioinformatics tools are required. Hence this study aimed to focus the genotype identification of the isolated dengue virus which is very important to find out the analogous strain circulating in the area. In this present study, we sequenced C-prM gene junction of one strain isolated from pediatric dengue virus patients. About 100 serum samples were collected among clinically suspected pediatric patients reporting to children's hospital from August 2011 to October 2011 in Chennai, Tamilndau, India. All the collected samples were analyzed for RT-PCR for identification of positive cases along with serotype prediction. Among the RT-PCR results, 10 cases were detected for dengue 1 which is predominant and 2 cases were dengue 3 serotype. Due to cost effectiveness, one predominant serotype was sequenced to identify the genotype of dengue virus to predict the dengue virus evolution. The Sequence of the isolate were also published in NCBI and the Phylogenetic tree were drawn with the help of appropriate Bioinfomatic tools. Our study showed that Dengue 1 was dominant in positive samples of dengue virus infection. The Dengue 1 isolate sequenced in our study belong to the genotype III
Authors and Affiliations
Shanthi. G, Indu Purushothaman and Rajarajan . S
Keywords
Related Articles
- EP ID EP138878
- DOI -
- Views 71
- Downloads 0
Two Step Enzymatic Hydrolysis and Fermentation Strategy for Conversion of Acid Treated Hydrolysate of Corn Cob to Ethanol
To study effect of different treatments on sugarcane bagasse in ethanol production
ABSTRACT Bioconversion of lignocellulose biomass to ethanol is significantly hindered by the structural and chemical complexity of biomass, which makes these materials a challenge to be used as feedstocks for cellulosi...
Molecular cloning and expression of tea chitinase gene in Pichia pastoris
Comparative Studies on Callus Induction from Different Explants of Vanilla planifolia Andrews
Antimicrobial and anticancer activity and DNA fingerprinting of extracts from Red Sea marine fungal symbiotes.
ABSTRACT Marine microorganisms have become an important source of pharmacologically active metabolites. More specifically, fungi from the marine environment have shown great potential as suggested by the diversity of...