Phylogenetic analysis of HIV-1 pol gene: first subgenomic evidence of CRF29-BF among Iranian HIV-1 patients
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2014, Vol 4, Issue 0
Abstract
Objective: To identify the dominant subtype among the HIV-1 strains circulation in Iran. Methods: In this cross sectional study 100 HIV positive patients participated. HIV-1 RNA was extracted from plasma. RT nested-PCR was performed and the final products were sequenced and phylogenetically analyzed; reference sequences were downloaded from Los Alamos, aligned with Iranian pol sequences in the study and analyzed by neighbor-joining method. Results: The results of the phylogenetic analysis showed that HIV-1 subtype CRF-35AD was the dominant subtype among HIV-1 infected patients in Iran; this analysis also suggested a new circulating recombinant form that had not previously been identified in Iran: CRF-29BF. Conclusions: The impact of HIV diversity on pathogenesis, transmission and clinical management have been discussed in different studies; therefore, analyses of HIV genetic diversity is required to design effective antiretroviral strategies for different HIV subtypes.
Isolation of pathogenic microorganisms from burn patients admitted in Dhaka Medical College and Hospital and demonstration of their drugresistance traits
Objective: To isolate and quantify the microflora from the burn patients admitted in the Division of Plastic Surgery and Burns outdoor patients in Dhaka Medical College Hospital, Bangladesh. Methods: Thirty wound surface...
Additional records of vector mosquito diversity collected from Al Khor district of North-eastern Qatar
Objective: To survey mosquito diversity in the north-eastern area, which includes the most important gas industrial city in Qatar, and to investigate the potential mosquitoes for transmitting diseases. Methods: A study w...
The seven-year longitudinal study on relapsing fever borreliosis in Western Iran
Objective: To determine the epidemiologic and clinical features of tick borne relapsing fever in Kurdistan Province during 2000-2006. Methods: In this cross-sectional clinic-based study, target group was all population o...
Development of a multiplex polymerase chain reaction protocol for the simultaneous detection of Salmonella enterica serovar Typhi and Class 1 integron
Objective: To develop a multiplex polymerase chain reaction (PCR) protocol for the simultaneous detection of Salmonella enterica serovar Typhi (S. Typhi) and Class 1 integron, so as to aid rapid diagnosis of S. Typhi cas...
Presbycusis and auditory brainstem responses: a review
Age-related hearing loss or presbycusis is a complex phenomenon consisting of elevation of hearing levels as well as changes in the auditory processing. It is commonly classified into four categories depending on the cau...