Phylogenetic analysis of HIV-1 pol gene: first subgenomic evidence of CRF29-BF among Iranian HIV-1 patients
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2014, Vol 4, Issue 0
Abstract
Objective: To identify the dominant subtype among the HIV-1 strains circulation in Iran. Methods: In this cross sectional study 100 HIV positive patients participated. HIV-1 RNA was extracted from plasma. RT nested-PCR was performed and the final products were sequenced and phylogenetically analyzed; reference sequences were downloaded from Los Alamos, aligned with Iranian pol sequences in the study and analyzed by neighbor-joining method. Results: The results of the phylogenetic analysis showed that HIV-1 subtype CRF-35AD was the dominant subtype among HIV-1 infected patients in Iran; this analysis also suggested a new circulating recombinant form that had not previously been identified in Iran: CRF-29BF. Conclusions: The impact of HIV diversity on pathogenesis, transmission and clinical management have been discussed in different studies; therefore, analyses of HIV genetic diversity is required to design effective antiretroviral strategies for different HIV subtypes.
Ovarian nematode (Nematoda: Philometra sp.) infestation on Pseudorhombus triocellatus (Paralichthydiadae)
Objective: To study the occurrence, description and prevalence of the Philometra sp. infestation in ovaries of Pseudorhombus triocellatus from two different landing centers in Tamil Nadu of southeast coast of India for a...
Interferon-γ +874A/T polymorphism associated with Toxoplasma gondii seropositivity in HIV patients
Objective: To investigate the association of polymorphisms in genes that code for interferon-γ (IFN-γ) and interleukin-10 (IL-10), which play important roles in Toxoplasma gondii (T. gondii) infection, with the occurrenc...
Non tuberculosis mycobacteria isolates among new and previously treated pulmonary tuberculosis patients in Nigeria
Objective: To determine the prevalence of non tuberculosis mycobacteria (NTM) among new and previously treated tuberculosis (TB) patients in Nigeria. Methods: It was a retrospective study. A total of 102 sputum smear pos...
Hydatidosis in human and slaughtered herbivores in Mazandaran province, northern Iran
Objective: To determine the rate of hydatid cyst in hospitalized patients and slaughtered animals in Mazandaran province, northern Iran. Methods: In this descriptive study, files of 34 hospitalized patients who underwent...
Protective effect of CSN1S2 protein of goat milk on ileum microstructure and inflmmation in rat-CFAinduced rheumatoid arthritis
Objective: To observe the protective effect of goat milk alpha (S2)-casein (CSN1S2) protein on ileum microstructure and inflammation in rat-complete Freund’s adjuvant-induced rheumatoid arthritis model. Methods: Twenty f...