Phylogenetic genetic analysis of porcine parvovirus 1 epidemic strains in Henan
Journal Title: Journal of Henan Agricultural University - Year 2023, Vol 57, Issue 2
Abstract
[Objective] The study was conducted to monitor the variation of porcine parvovirus 1 in Henan Province. [Method] The positive samples of aborted fetuses from newborn sows in Hebi City and Anyang City, Henan Province were collected. Two PPV1 epidemic strains were identified by PCR method. After complete genome sequencing, the resulting two strains were named CH/HN-H/2021 and CH/HN-3/2021 respectively. Through homology and genetic evolution tree construction, the genetic evolution of PPV1 complete genome, NS1 gene and VP2 gene was analyzed, and the amino acid variation of NS1 protein and VP2 protein was compared and analyzed. [Result] The nucleotide homology of the two sequences was 99.9%, while the complete genome nucleotide homology with 46 domestic and international strains was 93.5%-99.9%. The NS1 gene nucleotide homology was 98.6%-99.9%, the amino acid homology was 98.2%-99.9%, the homology of VP2 gene was 98.4%-99.9%, and the amino acid homology was 98.3%-99.9% indicating that the strains identified in this study showed genetic and evolutionary stability. The strains identified in this study have the closest genetic relationship with Hunan and Jilin strains, but have a far genetic relationship with the original strains in Henan. There is a situation that the strains identified in this study and the original strains in Henan are prevalent at the same time. In the analysis of amino acid variation of NS1 protein and VP2 protein, it was found that the mutation point of NS1 protein has 6 mutations and VP2 protein has 7 mutations, all within the range of classical mutation sites, in accordance with the conservatism of PPV1 genetic variation. The gene in the posterior non-coding region of the identified strain VP2 gene was missing 127 nt compared to NADL-2. [Conclusion] The strains identified in this study showed genetic and evolutionary stability, and there were new and old PPV1 strains prevalent at the same time in Henan Province.
Authors and Affiliations
Xiya XU, Chenmeng DING, Yawei SUN, Ziwei HAN, Jiangkun QI, Chenzhe LÜ, Mengmeng SHI, Ziyi GUO, Mengmeng DENG, Lu CHEN
Keywords
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- EP ID EP769190
- DOI 10.16445/j.cnki.1000-2340.20230104.002
- Views 5
- Downloads 0
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