Phytochemical Analysis and Antimicrobial Screening of PsorospermumsenegalenseLeaves Extract
Journal Title: Scholars Academic Journal of Biosciences - Year 2017, Vol 5, Issue 6
Abstract
The antibacterial activity of the hexane, dichloromethane, etyl acetate and methanol extracts of psorospermumsenegalense leaf belonging to the family Hypericaceae, was determined in vitro, using well diffusion and minimum inhibitory concentration (MIC) determination method against nine human pathogenic bacteria: (klebsiella pneumonia, Staphylococcus aureus, proteus vulgaris streptococcus pneumonia, vancomycin resistant enterococci, Bacillus subtilis, Escherichia coli, pseudomonas flourescense and streptococcus pyogenes,) and three human pathogenic fungi (candida tropicalis, candida krusei and Candida pseudotropicalis). The hexane, dichloromethane, ethylacetate and methanol extracts displayed a potential antibacterial activity against five out of the nine tested bacteria: (Staphylococcus aureus,, streptococcus pneumonia, Bacillus subtilis, Escherichia coli, and streptococcus pyogenes,)and two out of three tested fungi (candida tropicalis, and Candida pseudotropicalis)The ethyl acetate extract exhibited the highest zone of inhibition (28 mm) and MIC(3.25mg/ml) against Streptococcus pyogenes. The highest inhibition of fungal growth (27mm) and MIC (3.25mg/ml) was recorded against C. pseudotropicalis with ethyl acetate extract. The hexane extract exhibited the lowest zone of inhibition (18 mm) and MIC (15mg/ml) against streptococcus pneumoniae as well as C.Krusei and C.pseudokrusei. Phytochemical analysis showed the presence of carbohydrates, alkaloids Cardiac glycosides, steroids and triterpenes in all the four extracts, tannins and flavonoids were present in the ethyl acetate and methanol extracts. While Saponins and anthraquinones were absent in all the four extracts.The consequences of this investigation suggest that the extracts can be used to discover antibacterial agent for developing new pharmaceuticals to control studied human pathogenic bacteria and fungi responsible for severe illness.
Authors and Affiliations
Momoh, H, Dambata M. B, Yaau Datti
Keywords
Related Articles
- EP ID EP398046
- DOI -
- Views 69
- Downloads 0
Butterflies (Insecta: Lepidoptera) of Ajinkyatara Fort, Satara, Maharashtra, India
Abstract: Surveys and photographic collection of butterflies in Ajinkyatara fort Satara Maharashtra, India. is carried out from 28th August 2011 to 30 January 2012 still 36 species of butterflies belonging to 29 genera...
Infertility in The Philippines and Natural Procreative (Napro) Technology: A Commentary
Abstract: The latest retrievable data about infertility in the Philippines was made available to the public by Merck Serono, a private pharmaceutical company who commissioned the survey in 2011. The survey was conducted...
Antimicrobial Activities and Phytochemical Screening of the Leaf Extract of Mitracarpus Scaber
An evaluation of the anti-microbial activities of the leaf extract of Mitracarpus scaber shows that it has inhibitory effects on Pseudomonas aeruginosa, Proteus vulgaris, Escherichia coli, Staphylococcus aureus and Trych...
Adenosine Deaminase levels in Cerebrospinal fluid among Tubercular Meningitis patients
Abstract: Tuberculous Meningitis (TBM) is an endemic disease in developing countries, more so in low socio-economic status. CSF ADA (Cerebrospinal fluid Adenosine Deaminase) is thought to be released by T lymphocyte duri...
Lipid derangement and cardiovascular risk in female patients with major depressive disorder
Lipid disorder is well recognized risk factor for cardiovascular disease. The association of lipid derangement with depressive disorder has been a recent focus of research specially in female. The objective of this study...