Phytochemical Analysis and in vitro Antioxidant Activity of Fractions of Methanol Extract of Polyalthia longifolia var. Pendula Leaf
Journal Title: European Journal of Medicinal Plants - Year 2017, Vol 21, Issue 1
Abstract
This study was aimed to determine the antioxidant capacity of various fractions of crude methanol extract of Polyalthia longifolia leaves. The various fractions were obtained by successive liquid-liquid partitioning of the crude methanol extract into n-hexane, chloroform and ethyl acetate. The flavonoid and phenolic contents as well as the 2, 2- diphenyl-1-picrylhydrazyl scavenging activity and total reducing power of the crude methanol extract of Polyalthia longifolia leaves and its fractions were analyzed using standard methods. From the results obtained, ethyl acetate fraction had the highest flavonoid (571.30 mgQE/g) and phenolic (278.45 mgGAE/g) contents and the lowest IC50 values for 2, 2- diphenyl-1-picrylhydrazyl scavenging activity (˂ 1 µg/ml) and total reducing power (0.24 µg/ml). However, the water soluble fraction showed least values for flavonoid content (7.22 mgQE/g), phenolic content (48.66 mgGAE/g) and highest IC50 values for 2, 2- diphenyl-1-picrylhydrazyl scavenging activity (505.87 µg/ml) and total reducing power (47.99 µg/ml). The flavonoid content, phenolic content and total reducing power were in the order; ethyl acetate fraction > chloroform fraction > methanol extract > n-Hexane fraction > water soluble fraction. However, the inhibition of 2, 2- diphenyl-1-picrylhydrazyl free radical followed the order; ethyl acetate fraction > methanol extract > chloroform fraction > n-Hexane fraction > water soluble fraction. In general, the antioxidant activity of the ethyl acetate fraction was the most outstanding when compared with the crude methanol extract and other fractions. Having established the presence of significant concentrations of pharmaceutically important phytochemicals in the fractions of the methanol extract of the leaves especially the ethyl acetate, more evidence based studies such as analysis of specific chemical composition and in-vivo pharmacological potential of each fraction is imperative in order to expound more on the medicinal usability of the various fractions of the leaves.
Authors and Affiliations
Banji Adaramola, Oluchi Otuneme, Adebayo Onigbinde, Kunle Orodele, Abimbola Aleshinloye, Jegede David, Ayodele Ogunnowo, Aderiike Adewumi
Keywords
Related Articles
- EP ID EP327296
- DOI 10.9734/EJMP/2017/35002
- Views 115
- Downloads 0
Medicinal Plants from the Brazilian Savanna with Antibacterial Properties
Aims: Research on natural antimicrobials has increased due to the emergence of microbial strains resistant to conventional antibiotics. We investigated and report here the in vitro antibacterial properties of crude extra...
Antimicrobial Activities of Six Selected Plants against Multi-drug Resistant Staphylococcus aureus
The problem of microbial resistance is growing and the outlook for the success of antimicrobial drugs in the future is still uncertain. For a long period, plants have been a valuable source of natural products for mainta...
The Role of Monocarboxylate Transporters and Their Chaperone CD147 in Lactate Efflux Inhibition and the Anticancer Effects of Terminalia chebula in Neuroblastoma Cell Line N2-A
Aims: In the presence of oxygen, most of the synthesized pyruvate during glycolysis in the cancer cell of solid tumors is released away from the mitochondria to form lactate (Warburg Effect). To maintain cell homeostasis...
A Scientific Update on Passiflora foetida
Introduction: This review describes the importance of medicinal plant Passiflora foetida in the modern world. Between 190 countries, approximately 85% have dependency of traditional systems of Ayurveda, Homeopathy and Un...
Evaluation of Antistress Potential of Negundin A from Vitex negundo in Acute Stress Induced Mice
Study Background: The current study has been designed to evaluate the antistress activity of bioactive compound, Negundin A from hydroalcoholic extract of the leaves of Vitex negundo. An attempt has been made to isolate...