PHYTOCHEMICAL ANALYSIS AND IN VITRO ENZYME INHIBITORY ACTIVITY ESSAY OF EUPHORBIA HIRTA LINN. EXTRACTS
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2018, Vol 7, Issue 6
Abstract
In this study, in vitro analysis of on α-amylase and α-glucosidase inhibitory activity, total phenolic content (TPC), total flavonoid content (TFC) and phytochemical analysis of three different extracts (petroleum ether extract, chloroform extract and ethyl acetate extract) from crude extract of Euphorbia hirta Linn. (E.hirta) were determined using spectrophotometer and Fourier-transform infrared spectroscopy (FTIR) methods. α-amylase and α-glucosidase inhibitory activity of extracts were expressed as percentage of inhibition and IC50 values. Value in percentage of standard acarbose, crude, petroleum ether extract, chloroform extract and ethyl acetate extract at a concentration 10mg/ml were 94.69%, 55.06%, 16.55% and 40.97% inhibitory effects on α-amylase. Percentage inhibition of α-glucosidase at a concentration 10mg/ml were 54.91%, 76.67% and 98.39% presence for standard acarbose, crude extract and ethyl acetate extract in which petroleum ether extract and chloroform extract did not show inhibitory effects on α-glucosidase. Total phenolic content value of crude extract, petroleum ether extract, chloroform extract and ethyl acetate extract were 194.55 ± 0.82, 51.85 ± 3.12, 81.56 ± 1.72 and 214.21±2.53 mg of GAE/ g extract, expressed as gallic acid equivalents. Crude extract, petroleum ether extract, chloroform extract and ethyl acetate extracts showed total flavonoids 40.56 ± 7.27, 29.49± 1.66, 64.99 ± 2.60 and 91.69± 1.67 mg of RU/g extract, as rutin equivalents. FTIR spectra analysis of four extracts were comparable and showed the presence of alkaloids, saponins, polyphenols, terpenoids, steroids, flavonoids, quinones and terpenes. Two bioactive compounds that are 5-methoxybenzene-1,2,3-triol and 2-(2,4-dihydroxyphenyl)-3,6-dihydroxy-8-methoxy-4H-chromen-4-one were identified in ethyl acetate extracts by using column chromatography method, thin layer chromatography (TLC) and NMR spectroscopy.
Authors and Affiliations
Ly Le
Keywords
Related Articles
- EP ID EP627042
- DOI -
- Views 142
- Downloads 0
EXPLORING ANTI-HYPERGLYCEMIC POTENTIAL OF ALKALOID COMPOUNDS FROM CATHARANTHUS ROSEUS G. DON (L) AGAINST VARIOUS TYPE II DIABETES TARGETS BY IN SILICO VIRTUAL SCREENING
Background: Catharanthus roseus (L.) G. Don (C. roseus) is a prominent anticancer herb having high alkaloid content. Besides known anticancer effect, this plant has been also traditionally used as an effective anti-diabe...
EFFECTS OF COFFEE AND OVALTINE (MILO) ON BLOOD PRESSURE IN HEALTHY INDIVIDUALS
Background: The pressor or depressor effect of ovaltine (Milo) and coffee is significant for both public and scientific perspectives. They are most commonly consumed beverages around the world with limited and controvers...
THREE NEW UNREPORTED BASIDIOMYCOTA MACROFUNGI FROM JAMMU AND KASHMIR, INDIA
The present paper discusses the study of systematic survey for the exploration of agarics from North Kashmir. The area exhibits varied climatic and topographic conditions and provide an environment for the lavish growth...
REVIEW ON RUTA GRAVEOLENS
Bioassay-directed isolation of antifungal compounds from an ethyl acetate extract of Ruta graveolens leaves yielded two furanocoumarins, one quinoline alkaloid, and four quinolone alkaloids including a novel compound, 1-...
PROMETHAZINE AN ENVIRONMENTAL FRIENDLY REAGENT FOR NOVEL ESTIMATION OF HYPOCHLORITE IN ENVIRONMENTAL SAMPLES
A simple, rapid and accurate spectrophotometric method has been developed for the determination of hypochlorite using promethazine. The proposed method reports the reaction of hypochlorite with promethazine in acid mediu...