PHYTOCHEMICAL ANALYSIS AND IN VITRO ENZYME INHIBITORY ACTIVITY ESSAY OF EUPHORBIA HIRTA LINN. EXTRACTS
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2018, Vol 7, Issue 6
Abstract
In this study, in vitro analysis of on α-amylase and α-glucosidase inhibitory activity, total phenolic content (TPC), total flavonoid content (TFC) and phytochemical analysis of three different extracts (petroleum ether extract, chloroform extract and ethyl acetate extract) from crude extract of Euphorbia hirta Linn. (E.hirta) were determined using spectrophotometer and Fourier-transform infrared spectroscopy (FTIR) methods. α-amylase and α-glucosidase inhibitory activity of extracts were expressed as percentage of inhibition and IC50 values. Value in percentage of standard acarbose, crude, petroleum ether extract, chloroform extract and ethyl acetate extract at a concentration 10mg/ml were 94.69%, 55.06%, 16.55% and 40.97% inhibitory effects on α-amylase. Percentage inhibition of α-glucosidase at a concentration 10mg/ml were 54.91%, 76.67% and 98.39% presence for standard acarbose, crude extract and ethyl acetate extract in which petroleum ether extract and chloroform extract did not show inhibitory effects on α-glucosidase. Total phenolic content value of crude extract, petroleum ether extract, chloroform extract and ethyl acetate extract were 194.55 ± 0.82, 51.85 ± 3.12, 81.56 ± 1.72 and 214.21±2.53 mg of GAE/ g extract, expressed as gallic acid equivalents. Crude extract, petroleum ether extract, chloroform extract and ethyl acetate extracts showed total flavonoids 40.56 ± 7.27, 29.49± 1.66, 64.99 ± 2.60 and 91.69± 1.67 mg of RU/g extract, as rutin equivalents. FTIR spectra analysis of four extracts were comparable and showed the presence of alkaloids, saponins, polyphenols, terpenoids, steroids, flavonoids, quinones and terpenes. Two bioactive compounds that are 5-methoxybenzene-1,2,3-triol and 2-(2,4-dihydroxyphenyl)-3,6-dihydroxy-8-methoxy-4H-chromen-4-one were identified in ethyl acetate extracts by using column chromatography method, thin layer chromatography (TLC) and NMR spectroscopy.
Authors and Affiliations
Ly Le
Keywords
Related Articles
- EP ID EP627042
- DOI -
- Views 148
- Downloads 0
A STUDY TO DETERMINE TOTAL PHENOLIC CONTENT OF OPUNTIA FICUS-INDICA EXTRACTS AND THEIR ACTIVITY AGAINST SOME PATHOGENIC FUNGI
This study was carried out to determine total phenolic content Of cactus plant extracts (Opuntia ficus-indica) and their activity against some pathogenic fungi. The results of analysis of total phenolic content of methan...
INFECTIOUS ENDOCARDITIS REVEALED BY SPONDYLODISCITIS: A CASE REPORT.
The first describe association between spondylodiscitis and endocarditis was reported in 1965 by de Sèze et al., since then, many case reports and series have been published. The most common clinical picture of this asso...
TRAUMATIC LUMBAR FRACTURE ASSOCIATED WITH A THORACIC SPINAL CORD INJURY WITHOUT RADIOLOGIC ABNORMALITY: A CASE REPORT AND LITERATURE REVIEW.
A case of complete paraplegia due to a T8 spinal cord injury without radiologic abnormality (SCIWORA) associated with a L3 vertebral fracture is presented. SCIWORA is a rare injury, most frequently seen in the pediatric...
A RETROSPECTIVE STUDY IN LEBANON. THYROID-STIMULATING HORMONE AND ITS POSSIBLE ASSOCIATION WITH SERUM LIPIDS.
Thyroid gland diseases are a public health problem worldwide. Many disorders result from the imbalance in the regulation of thyroid hormones, these ranges from a small goiter to life threatening diseases, like thyroid ca...
A REVIEW ON MEDICINAL PROPERTIES OF LANTANA CAMARA LINN
Lantana camara is well known to cure several diseases and used in various folk medicinal preparations. In last few decades, scientist and researchers around the globe have elaborately studied the chemical composition of...