PHYTOCHEMICAL ANALYSIS, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY DETERMINATION OF PIPER NIGRUM
Journal Title: European Journal of Pharmaceutical and Medical Research - Year 2019, Vol 6, Issue 10
Abstract
To evaluate the phytochemical constituents, antibacterial and antioxidant scavenging activity of ethanolic Soxhlet and maceration extract of Piper nigrum fruit. In the present research, Soxhlet extraction and maceration extraction of Piper nigrum were carried out by using 95% ethanol as solvent. Phytochemical analysis of the important chemical constituents were carried out for both the ethanolic extracts. Antibacterial activity of Piper nigrum extract was determined by using well diffusion method and the zone of inhibition was measured. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were carried out subsequently. Antioxidant activity of Piper nigrum was determined by using DPPH radical scavenging assay and the IC50 values were determined. Total phenolic content was determined by the Folin-Ciocalteu method and expressed as mg Gallic acid per gram of plant material. For phytochemical analysis, both extracts showed positive results in all the tests except in the test for saponin, anthraquinone, glycoside and flavonoid. For the well diffusion test, positive results were seen in all the bacterial strains except for Escherichia coli. The MIC and MBC could not be determined accurately as the concentration used was too low. For antioxidant analysis, DPPH radical scavenging activity and total phenolic contents showed a positive correlation whereby the activity increases with the concentration. It is concluded that Piper nigrum exhibits antibacterial and antioxidant properties. It is concluded that Piper nigrum exhibits antibacterial and antioxidant properties.
Authors and Affiliations
Naeem Hasan Khan
Keywords
Related Articles
- EP ID EP674346
- DOI -
- Views 166
- Downloads 0
CAREER DECISION MAKING SELF-EFFICACY, GOAL STABILITY AND ACADEMIC ACHIEVEMENT AMONG UNIVERSITY STUDENTS
There is a gigantic interest in present era regarding career decision making, career selection and satisfaction that can be traced since the educational period when students are required to make choices for their career....
EVALUATION OF DENTAL PRESCRIPTIONS IN VARIOUS DENTAL HOSPITALS/CLINICS OF PUNJAB, PAKISTAN.
Objective: the aim of the study was evaluate the prescription and medication errors in dental prescriptions prescribed in various dental clinics/hospitals of Punjab, Pakistan. Material & methods: 150 pre-designed prescri...
SYNTHESIS AND ANTIPLASMODIAL EVALUATION OF A NEW TRIOXAQUINE
The ultimate objective of this work was to develop a novel antimalarial hybrid drug based on quinoline and trioxane pharmacophoric scaffold that is effective against drug-resistant malaria, especially in the face of emer...
TRENDS IN THE SEROPREVALENCE OF TRANSFUSION–TRANSMISSIBLE INFECTIONS (TTIS) AMONG POTENTIAL BLOOD DONORS IN A TERTIARY HEALTH FACILITY IN SOUTH–SOUTH NIGERIA
Background and Objectives Transfusion – transmissible infections still pose a formidable threat to blood transfusion safety in Sub-Saharan Africa owing to the endemicity of these infections in the region. Blood donors ca...
MORPHOLOGICAL CHARACTERISTICS OF METHODS FOR ASSESSING KI-67 IN INFILTRATING BREAST CANCER
The problem of breast cancer (BC) is relevant for modern oncology, which is associated with a pronounced increase in the incidence of women with this pathology and the search for new ways of treatment, in particular the...