PHYTOCHEMICAL AND ANTIOXIDANT CHARACTERIZATION OF THINNED IMMATURE CITRUS UNSHIU FRUITS

Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 12

Abstract

Objective: We aimed to evaluate the characterization of thinned immature Citrus unshiu fruits with regard to their phytochemical profile and antioxidant capacity.Methods: Determination of total phenolic, flavonoid, and carotenoid and ascorbic acid contents was done by UV-Visible spectrophotometry, whereas UPLC-mass detection was used for the analysis of individual flavanone (naringin, hesperidin, hesperetin, neohesperedine and narirutin) and flavonol (rutin). In addition, free radicals (DPPH, O2-, H2O2 and NO) scavenging assays were used to determine the antioxidant capacity.Results: Naringin, hesperidine, neohesperedine and narirutin were the main flavanones in all thinned immature Citrus unshiu fruits. The contents of total phenolic, flavonoid and carotenoid were more prevalent in immature fruits than the level found in mature fruits. All thinned immature Citrus unshiu fruits possess an evident antioxidant capacity. The immature Citrus extract concentrations providing 50% inhibition (IC50) for free radicals; 1.2-1.49 mg/ml for DPPH, 1.03-1.46 mg/ml for superoxide, 1.95-3.43 mg/ml for hydrogen peroxide and 1.64-3.45 mg/ml for nitric oxide was lower than those of mature Citrus extracts.Conclusion: Thinned immature Citrus unshiu fruits could be an economic and readily accessible source of natural antioxidants and as a possible food and pharmaceutical supplement.

Authors and Affiliations

Ji Hye Kim, Min Young Kim

Keywords

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  • EP ID EP575436
  • DOI 10.22159/ijpps.2017v9i12.22971
  • Views 53
  • Downloads 0

How To Cite

Ji Hye Kim, Min Young Kim (2017). PHYTOCHEMICAL AND ANTIOXIDANT CHARACTERIZATION OF THINNED IMMATURE CITRUS UNSHIU FRUITS. International Journal of Pharmacy and Pharmaceutical Sciences, 9(12), 293-297. https://europub.co.uk/articles/-A-575436