Phytochemical and Antioxidant Screening of Seed Extracts of Kenyan Custard Apple (Annona squamosa)
Journal Title: International Journal of Business and Management Invention - Year 2017, Vol 6, Issue 7
Abstract
Medicinal plants have been the mainstay of traditional herbal medicine amongst rural dwellers worldwide since antiquity for their preventive and curative properties. Investigation of plants for phytochemicals and medicinal activities is imperative for the discovery of prospective novel molecules for medicinal use. This current analysis was aimed at screening the seed extracts of Annona squamosa for the presence of phytochemicals and their antioxidant activities. Extraction of the active components was through organic solvents; methanol, dichloromethane and methanol: dichloromethane blend in Soxhlet extractor and concentrated in a rotary evaporator. Investigation of phytochemicals was through standard qualitative screening procedures while antioxidant activities of the extract were evaluated through the radical scavenging effect of DPPH, H2O2 and the FRAP assay. Methanol was the most effective solvent in capturing phytochemicals including tannins, alkaloids, flavonoids, fixed oils and phenols. Dichloromethane captured only alkaloids and fixed oils. The antioxidant data obtained was assayed against that of the standard (Ascorbic acid). The methanolic extract was found to be the most potent extract with highest antioxidant activities followed by methanol: dichloromethane blend while dichloromethane portrayed the least potency. The highest DPPH radical scavenging was at 500mg/ml, while for H2O2 was at 1mg/ml while the highest FRAP activity was at 1mg/ml. Methanolic extracts were the most potent while dichloromethane extracts showed the least DPPH and H2O2 radical scavenging activities. Phenols appear have significantly contributed towards the antioxidant activity of the methanolic extracts and the alkaloids in dichloromethane extracts. Most of the inedible parts of commonly consumed fruits have not been studied for their antioxidant activity but the current assay demonstrates that seeds may be a promising source of antioxidants. This study shows that the seed extracts of Annona squamosa may be utilised therapeutically in the management of oxidative stress related disorders.
Authors and Affiliations
Stephen K. Mwihia, Mathew P. Ngugi, John M. Maingi
Keywords
Related Articles
- EP ID EP406472
- DOI -
- Views 159
- Downloads 0
An Identifying Organization Strategy of City Laundry in Ipoh, Perak
The way in which we plan and produce buildings today will influence our energy consumption in the future. This paper identifies an organization strategy of one of the self-service laundry that is situated in Ipoh, Perak,...
‘Mission Possible: Financial Transparency in India’A Thoughtful Critical Theoretical Study on Financial TransparencyOf Indian Premier Non-Corporate Non-Financial Institutions
Transparency & Accountability are the two terms quite familiar to the research world. The pertinent point is how India would respond to it in a globalised world? Author attempts to find & analyse India’s journey towards...
Role of Informal Financial Banking Services in Economic Empowerment of Rural Women through Self-Help Groups Affiliated To Joyful Women Organization in Kericho County, Kenya
Women all over the world contribute a lot to the national agricultural output and family food security, and several studies from Sub Saharan Africa shows that rural women are credit constrained than men of equivalent soc...
Vascular endothelial growth factor (VEGF), cartilage oligomeric protein (COMP) and matrix metalloproteinase 3 (MMP-3) as serum biomarkers in psoriatic arthritis
Introduction. Psoriatic arthritis (PsA) is a chronic inflammatory musculoskeletal disorder characterized by axial and peripheral joint involvement as well as extra-articular manifestations such as skin and nail disease,...
Financial Leverage and Firm Performance –An Empirical Study of Select Public Sector Undertakings listed at BSE, India
In the process of profit magnification, operating leverage and financial leverage play a vital role in the organization. Operating leverage magnifies the effect of change in sales on operating profit as measured by Earni...