PHYTOCHEMICAL EVALUATION AND IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY EFFECTS OF CLERODENDRUM SERRATUM ROOTS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 8
Abstract
Objective: To evaluate in vitro free radical scavenging and anti-inflammatory effects of extract and fractions of Clerodendrum serratum roots along with its phytochemical analysis.Methods: The crude 70% methanolic extract (MECSR) and ethyl acetate (EFCSR), n-butanol (BFCSR) and residual aqueous fractions (AQFCSR) of C. serratum roots were prepared and analyzed for qualitative and quantitative phytochemical study using reported methods. The in vitro anti-inflammatory effects were studied using protein denaturation and proteinase inhibitory assays whereas in vitro free radical scavenging effects were established using DPPH (1,1-diphenyl-2-picrylhydrazyl), modified 2,2’-azinobis (3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) and trolox equivalent antioxidant capacity (TEAC) assays.Results: The qualitative and quantitative phytochemical studies of crude extract and fractions showed the presence of phenolics, flavonoids, saponins, and carbohydrates. All analyzed samples showed dose-dependent anti-inflammatory and free radical scavenging effects in the studied in vitro assays. The IC50 values of MECSR, EFCSR, BFCSR and AQFCSR for scavenging the DPPH and ABTS radical ranged from 12.52±2.21 to 200.47±2.84 µg/ml and 18.12±1.76 to 216.08±1.90 µg/ml, respectively, while that of protein denaturation and proteinase inhibition assays were ranged from 70.76±1.40 to 110.13±1.63 µg/ml and 76.66±2.02 to 116.55±2.19 µg/ml.Conclusion: Among the analyzed samples, the EFCSR showed significantly higher free radical scavenging and anti-inflammatory effects in studied assays. The observed activities might be attributed to the higher content of polyphenols present in EFCSR fraction of roots. The present study will provide scientific data to reinforce the traditional claims of roots for treating inflammation, pain and other oxidative stress related diseases.
Authors and Affiliations
Niyati S. Acharya, Jagruti J. Patel
Keywords
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