Phytochemical profile and antimicrobial potential of methanolic extracts of bark and leaf of Quassia indica (Gaertn.) Nooteb.
Journal Title: UNKNOWN - Year 2017, Vol 6, Issue 5
Abstract
The study comprises the results of phytochemical analysis and antimicrobial evaluation of extracts from bark and leaf of Quassia indica (Gaertn). Nooteb. – a medicinal plant used in traditional healing owing to its analgestic, antiinflammatory, antifeedant and antimicrobial properties. A preliminary qualitative analysis was carried out successively in five different solvents with increasing order of polarity-Petroleum ether, Chloroform, Ethyl acetate, Methanol and Water to document the nature and yield of phytochemicals. The extracts were evaluated for antimicrobial effect using two strains of bacteria – Escherichia coli and Staphylococcus aureus and fungi – Aspergillus niger and Candida albicans. Among solvents methanol and water were found as effective extractants in which most of the secondary metabolites - alkaloids, flavonoids, terpenoids, phenolics, tannins, phytosterols were released. Quantitative analysis of the methanolic and aqueous extracts was carried out to estimate the quantity (mg/g tissue) of the phytoconstituents. The alkaloid content was much higher in leaf extract (5.7 mg/g) than in bark (3.5 mg/g). The phenolic content expressed as mg/ g GAE was determined in the methanolic extract, bark (24.38) > leaf (10.44) and the aqueous extract does not show much phenolic content. Flavonoid were maximum in methanolic leaf extract (1.085 mg/g) and minimum in aqueous bark extract (0.305 mg/g) and the terpenoid content was detected in methanolic extracts of leaf (0.4016 mg/g) and bark (0.4224 mg/g). The leaf extract indicated more tannin content (1.536 mg/g) than bark (1.328 mg/g). Evaluation of antimicrobial activity suggested leaf extract as an effective antibacterial and antifungal agent at a concentration of 1000 g/ml with inhibition zones- 24 mm (S.aureus), 22 mm (E.coli) and 14 mm (A.niger), 14 mm(C. albicans). The bark extract was comparatively lesser efficient in resisting microbial growth (E. coli – 20 mm; S. aureus – 22 mm; A. niger – 12 mm; C. albicans – 10 mm).
Authors and Affiliations
Anusha P, Sudha Bai R
Keywords
Related Articles
- EP ID EP451372
- DOI -
- Views 102
- Downloads 0
Medicinal plants used by tribal and rural community of Sata Pokhran area of tehsil Pampore of district Pulwama, Jammu and Kashmir-India
Man has used plants since time immemorial to alleviate sufferings and ailments. The present study attempts to explore and document the medicinal plants used against several diseases by the tribal and rural community of S...
Standardization of Haridradi churna- physicochemical assay and HPTLC profile
Haridradi churna, a polyherbal preparation containing Haridra (Curcuma longa Linn.), Devadaru (Cedrus deodar Roxb), Lodhra (Symplocos racemosa Roxb), Priyangu (Callicarpa macrophylla Vahl) and Yastimadhu (Glycyrrhiza gla...
Pharmacognostical and Phytochemical Evaluation of Pilla Shukra Nashaka Anjana Varti: An ocular Ayurvedic Formulations
Pilla Shukra Nashaka Anjana Varti is an important Ayurvedic formulation containing Haritaki (Terminalia chebula Retz.), Amalaki (Emblica officinalis Gaertn.), Bibhitaki (Terminalia bellerica Roxb.), Katuki (Picrohriza ku...
Ingredient identification and phytochemical evaluation of Shalmali kantaka cream
Background: In any system of medicine the drug identification is the most essential task prior to any treatment plan. In Ayurved it has more significance as there are various plants available with similar characteristics...
Toxicity evaluation and protective effect of Rhus longipes Engl. leaf extract in paracetamol induced oxidative stress in wister rats
Aim: Acute toxicity and protective effect of ethanol leaf extract of Rhus longipes Engl. against Paracetamol induced oxidative stress was investigated. The LD50 of the leaf extract was determined using up and down techni...