Phytochemical Screening and Anti-diabetic Evaluation of Citrus sinensis Stem Bark Extracts
Journal Title: International Journal of Biochemistry Research & Review - Year 2017, Vol 17, Issue 2
Abstract
Aim: Diabetes Mellitus remains amongst the highest cause of mortality in the world. This study was carried out to determine the phytochemical composition of stem bark extracts of Citrus sinensis and study their anti-hyperglycaemic activities as well as their modulatory effects on starch and sucrose digestion on normal Wistar rats. Methodology: Stem bark Aqueous (AqE), Ethanolic (EtE), Hydroethanolic (HEE) extracts of C. sinensis were prepared. The content of polyphenols, flavonoids, alkaloids was determined. Anti-hyperglycaemic activity was evaluated in vivo by oral glucose tolerance test (OGTT) for 120 minutes. Wistar male rats of 180-200 g were used. After an overnight fast, their glycaemia were measured (T0). Rats were then distributed into five groups of six each: Control (water), Tests (400 mg/kg of each (AqE, EtE, HEE) extracts), Reference (4 mg/kg B. W., glibenclamide). Thirty minutes later, an overload of 2 g/kg B. W. of glucose was administered to all the groups. Glycaemia was measured by tail pricks: 30, 60, 90, 120 minutes after glucose administration. Sucrose (2 g/kg B. W.), starch (1 g/kg B. W.) and acarbose in co-administration were used as substrates for digestion in a protocol similar to OGTT. Results: EtE was very rich in polyphenols (435.56 ± 3.85 µg of eq catechin/mg). Area under the Curves, AUC (mg.min.dL-1, P=.05) were: 4015, 786.6, 1163 for PC, AqE group, Reference respectively and confirmed that AqE was more efficient than glibenclamide. AqE also showed moderate efficacy with sucrose (841.2 vs 1172 mg.min/dL, P=.05) and starch digestion (892.8 vs 1148; P=.05) compared to positive controls. Conclusion: Therefore, stem bark aqueous extract of C. sinensis is able to reduce postprandial glycaemia by slowing down the absorption of glucose, challenging the efficacy of glibenclamide,an anti-diabetic drug. Also, AqE has moderate efficacy on starch and sucrose digestion. AqE could thus be further explored for its potency in the treatment of diabetes mellitus.
Authors and Affiliations
Azantsa Kingue Gabin Boris, Djuikoo Nouteza Imelda, Kuikoua Tchetmi Wilfried, Takuissu Guy, Judith Laure Ngondi, Julius Oben
Keywords
Related Articles
- EP ID EP316995
- DOI 10.9734/IJBCRR/2017/34051
- Views 155
- Downloads 0
Ethanol Extract of Acalypha wilkesiana Muel Arg Leaves Ameliorates Paracetamol-induced Hepatotoxicity in Rats
Objective: The effect of ethanol leaf extract of A. wilkesiana on the liver of paracetamol- induced hepatotoxicity was investigated in wistar albino rats. Study Design: Animal experimental study. Place of Study: Departme...
Growth Factor Receptors and Liver Injury
Aims: The aim of this study is to investigate the expression pattern of transforming growth factor β receptor I(TGFβRI) and fibroblast growth factor receptor 3 (FGFR3) at the different stages of liver injury including ac...
Comparative Investigation of the Effects of the Commonly-consumed Aqueous Extracts of Hibiscus sabdariffa (Zobo Drinks) on Body Weight, Glucose Level and Lipid Profile Using Normal Wistar Albino Rats
Aim: Investigating and comparing the effect of administration of different preparations of the commonly-consumed Hibiscus sabdariffa (Zobo) drinks on body weight, glucose level and lipid profile. Study Design: Animal mod...
Characterization and Expression Analysis of WRKY Transcription Factors in Groundnut (Arachis hypogaea L.)
Groundnut (Arachis hypogaea L.), is an important subsistence oil yielding crop of the semi-arid tropics and often exposed to several environmental cues (high temperature, drought & heavy metal). The WRKY transcription fa...
Cytotoxicological Response of Zea mays to Crude Oil: The Ecological Effects of Exposure to Contaminants
Cytotoxicity of different concentrations of crude oil on Zea mays a widely cultivated crop in Nigeria was investigated for 21 days, using physicochemical and cytotoxicity assay. The experiment was conducted in green-hous...