Phytochemical Screening and Antimicrobial Activities of the Fractionated Leaf Extract of Combretum Racemosum
Journal Title: Scholars Academic Journal of Pharmacy - Year 2014, Vol 3, Issue 6
Abstract
The phytochemical and anti-microbial properties of the leaves of Combretum racemosum were examined in this research work. The phytochemical screening of this plant leaves using ethanol, propanol and water reveal the presence of antraquinone, tannin, steroids, cardiac glycosides, saponin, reducing sugars, flavonoids, terpenoids and phlobatannin at various degrees of concentrations. The leaf extract also shows antibacterial and antifungal activities. Minimum inhibitory concentration for the ethanolic extract with Staphylococcus aureus, Psudomonas aeruginosa, Proteus vulgaris, Escherichia coli and Salmonella typhi range from 7.8125-31.25mg/ml; for the propanolic extract Minimum inhibitory concentration gave a range from 31.25-62.3mg/ml while for the aqueous extract the Minimum inhibitory concentration ranged from 15.625-125.0mg/ml respectively for the different bacteria. The ethanolic extract on Candida albicans gave a Minimum inhibitory concentration of 15.625mg/ml. Mycelial radial growth using the ethanolic and aqueous extract on Aspergillus niger and Rhizopus spp were examined. A percentage inhibition which ranged from 31.75-65.5% were obtained for Aspergillus niger for ethanolic extract and 7.7-9.6% for the aqueous extract while for Rhizopus spp, the percentage inhibition for ethanolic extract gave a range from 47.57-60.69% and 12.17-15.39% for the aqueous extract. The aqueous extract on Aspergillus niger and Rhizopus spp had no much significant activity on the plant extract. The hydrolysed alkaloid fraction obtained using the Stas Otto method gave good bacterial activity on all bacteria used and on Candida albicans and this was concentration dependent. Thus the presence of these secondary metabolites and the antimicrobial activities could be responsible for the potency of Combretum racemosum as a medicinal plant for the treatment of bacterial and fungal infections. Keywords: Combretum racemosum, Antimicrobial activities, Traditional medicine, Extract, Mycelial radial growth.
Authors and Affiliations
M. Oghenejobo ,, B. U. S. Oghenejobo, K. E. Uvieghara, E. Omughele ,
The Effect of Feeding Commercial Oil of Black Cumin in Sheep
This study investigated the effects of feeding commercial oil of the Black cumin (seeds of Nigella sativa) on the levels of total cholesterol and the body weights in sheep. Twelve male cross breed sheep were divided indi...
Detection and Estimation of Phenolic Acid and Flavonods in Leaves of Cadaba indica Lam by High Performance Thin Layer Chromatography
Abstract: Cadaba indica Lam. is one of the medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases of mankind. The present study mainly aimed to estimate the content of major...
An Overview of Current Treatments of Migraine
Abstract: The name ‘migraine’ has its origin from the Greek word hemicrania, which means ‘half of the head’, indicating the main feature of migraine i.e it affects only half portion of the head. It is the most widespread...
Nanosuspension in Drug Delivery-A Review
Nanotechnology is the science that deals with the process that occurs at molecular level and of nano length scale size. Nano refers to the particle size range of 1-1000 nm. Nanosuspensions are coming under nanotechnology...
Chemopreventive Influence of Kolaviron on 1,2-Dimethylhydrazine Induced Plasma Carbonyl Content and Non-Enzymatic Antioxidant Status in Rat Colon Carcinogenesis
Colon cancer is the second leading cause of cancer death worldwide with diet playing a prominent role in disease initiation and progression. We have investigated the chemopreventive efficacy of kolaviron on plasma protei...