PHYTOCHEMICAL SCREENING AND HISTOLOGY APPEARANCE OF ACUTE ORAL TOXICITY STUDY ON ETHANOL EXTRACT OF PSIDIUM GUAJAVA LINN. FRUIT IN MICE
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 1
Abstract
Objective: The main objectives of the research are to investigate the phytochemical screening, histology appearance, and safety of acute oral toxicity study on the extract of the fruit of Psidium guajava Linn. in mice.Methods: Mice that were administered by oral feeding with different and controlled dose were divided into three groups, with dose limits of both 2000 and 5000 mg/kg b.w. We analyzed the P. guajava Linn. extract with specific methods before treating the subject. The methods were followed with acute oral toxicity study of Up-and-Down Procedure Organization for Economic and Development 425. The mice were then observed for signs and symptoms of toxicity. In addition, toxicity in the liver and kidney was analyzed through histology observation.Results: Phytochemical screening revealed the presence of flavonoids, quinone, triterpenoid/steroid, tannins and saponins, and the absence of alkaloids. We found that the treatment with 2000 and 5000 mg/kg b.w. of the extract did not show any differences in body weight changes, number of hepatocyte in liver, and podocyte in kidney compared with control (*p>0.05). Moreover, we noticed all mice lived and were healthy during observation.Conclusion: Our finding indicates that the extract of the fruit of P. guajava Linn. is safe and it was not toxic to the liver and kidney.
Authors and Affiliations
Nur Atik, Iskandar Muda, Andri Reza Rahmadi, Achadiyani Achadiyani, Diah Dhianawaty Djunaedi
Keywords
Related Articles
- EP ID EP607160
- DOI 10.22159/ajpcr.2019.v12i1.30118
- Views 129
- Downloads 0
BERBERINE HYDROCHLORIDE COULD PROVE TO BE A PROMISING BULLET AGAINST CLOSTRIDIUM DIFFICILE INFECTION: A PRELIMINARY STUDY FROM SOUTH INDIA
Objective: Recurrent Clostridium difficile infection (CDI) and the emergence of strains with reduced susceptibility to metronidazole and vancomycinwarrants alternative therapy. Hence, we tested the potential efficacy of...
METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CLOTRIMAZOLE, MICONAZOLE NITRATE, AND TINIDAZOLE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD IN TABLETS
Objective: The aim of the study is to develop and validate a high-performance liquid chromatographic method for the simultaneous determination of clotrimazole, miconazole nitrate, and tinidazole tablet dosage forms. Mat...
CYTOTOXIC ACTIVITY OF ANREDERA CORDIFOLIA LEAF EXTRACT ON HELA CERVICAL CANCER CELLS THROUGH p53-INDEPENDENT PATHWAY
 Objective: This study investigated cytotoxic activity and apoptosis induction of Anredera cordifolia leaf extract on cervical cancer cells.Methods: A. cordifolia leaf extract was prepared by maceration using 70% ethano...
A REVIEW ON NASAL DRUG DELIVERY SYSTEM AND ITS CONTRIBUTION IN THERAPEUTIC MANAGEMENT
Nasal drug delivery has received a great deal of attention as a convenient, reliable, and promising method for the systemic administration of drugs. It is especially for those molecules which are ineffective orally and o...
PREPARATION, PHYSICAL CHARACTERIZATION, AND PHARMACOKINETIC STUDY OF DOCETAXEL NANOCRYSTALS
Objective: The main objective of this study was to prepare and evaluate the nanocrystal formulation of docetaxel. Methods: Docetaxel nanocrystals were formulated to improve the water solubility. Docetaxel nanocrystals w...