PHYTOCHEMICAL SCREENING AND IN VITRO ANTILISTERIAL ATTRIBUTES OF DIFFERENT AQUEOUS AND ETHANOLIC LEAF EXTRACTS
Journal Title: Annals of Mechnikov Institute - Year 2019, Vol 0, Issue 3
Abstract
Introduction. Listeria monocytogenes represents the Listeria species most commonly associated with disease in humans. The majority (99%) of the infections caused by L. monocytogenes are food-borne being ingestion of contaminated food especially contaminated ready-to-eat food products that do not undergo subsequent reheating. This organism has great economical implications in the food industry due to recalls of contaminated food products and temporary shutdown of many food processing plants. There has been lots of interest recently in the role of complementary and alternative medicines for the treatment of various acute and chronic diseases. The revival of interest in the use of African medicinal plants by many developing countries and the World Health Organization (WHO) has led to intensified efforts to explore the numerous plants with medicinal importance. A large number of phytochemicals belonging to several chemical classes have been shown to have inhibitory effects on all types of microorganisms in vitro. Knowledge of the chemical constituents of plants and their anti-Listeria ability is desirable because such information will be value for synthesis of complex chemical substances. The aim of this work was to study the phytochemical qualitative profiles and in vitro antilisterial attributes of aqueous and ethanolic leaf extracts from several plants against earlier documented Ready To Eat (RTE) associated multi antibiotic resistant L. monocytogenes strains. Materials and Methods. The examined aqueous and ethanolic leaf extracts were derived from Psidium guajava (guava), Zingiber officinale (Ginger), Dacryodes edulis (African pear), Citrus aurantifolia (Lime), Funtumia elastica (silkrubber), Vernonia amygdalina (Bitter leaf), Cassia alata and Moringa oleifera (horseradish tree). The respective L. monocytogenes strains utilized were; LMSN 70, LMEW 94 and LMMP 104. Invitro assay of aqeous and ethanol estracts of plants was assayed by agar well diffusion assay. Results and discussion. Alkaloids, saponins, steroids, terpenoids, cardiac glycoside, reducing sugar, phenolics, resins, flavonoids, and tannins were detected in Z. officinale ethanolic leaf extract whilst cardiac glycoside was absent in ethanolic leaf extracts of D. edulis, C. aurantifolia and F. elastica. Alkaloids, saponins, steroids, terpenoids, cardiac glycoside, reducing sugar, phenolics, resins, flavonoids, and tannins were detected in crude aqueous leaf extracts of Z. officinale and M. oleifera.F. elastica ethanolic leaf extract displayed the highest antilisterial potential at the least concentration; 100mg/ml whilst amongst the aqueous extracts, V. amygdalina leaf extract exhibited maximal antilisterial activities comparable with antilisterial inhibitory growth zones elicited by the respective ethanolic extracts with the exception of M. oleifera extracts. Conclusion. Further studies aimed at the fractionation of the respective crude extracts especially F. elastica and exposing the respective multi antibiotic resistant food borne L. monocytogenes strains to these fractionated leaf extracts should be conducted.
Authors and Affiliations
Daniel O. Ebakota, Onilude Abiodun Anthony, Obayagbona Omoregbe Nosa
Keywords
Related Articles
- EP ID EP645494
- DOI 10.5281/zenodo.3469444
- Views 161
- Downloads 0
SUBSTANTIATION OF OVERCOMING OF ANTIBIOTIC RESISTANCE IN ACINETOBACTER BAUMANNII CLINICAL STRAINS BY USAGE OF DECAMETHOXINUM
Introduction. Non-fermenting Gram-negative bacilli are known as one of the most frequent causative agents of hospital-acquired infections. Acinetobacter baumanii, as causative agent of infection complications of differen...
BIOMARKERS FOR SYSTEMIC SCLEROSIS - TOOLS FOR DIAGNOSIS AND TREATMENT
Introduction. Systemic sclerosis (SSc) is an autoimmune disease characterized by skin and internal organ fibrosis with prior vascular and immune dysfunction. Depending on the cutaneous fibrosis degree, SSc is divided int...
HOMOLOGY MODELING AND MOLECULAR DYNAMICS STUDY OF MYCOBACTERIUM TUBERCULOSIS UREASE
Introduction. M. tuberculosis urease (MTU) is an attractive target for chemotherapeutic intervention in tuberculosis by designing new safe and efficient enzyme inhibitors. A prerequisite for designing such inhibitors is...
SECRETORY IMMUNOGLOBULIN A AND ITS ROLE IN FORMATION OF CLINICAL COURSE OF SHIGELLOSIS IN CHILDREN INFECTED WITH HELICOBACTER PYLORI
Introduction. Secretory immunoglobulin A (sIgA) is a crucial factor in protection of the gastrointestinal (GI) tract mucosa directly providing the first line of defense of the intestine from the impact of foreign antigen...
STUDY OF THE INFLUENCE OF PHARMACEUTICAL FACTORS ON THE OPTIMIZATION OF THE RELEASE OF BIOLOGICALLY ACTIVE SUBSTANCES UPON RECEIPT THE WATER EXTRACTS FROM GYNECOLOGICAL PLANT MEDICAL COLLECTION
Introduction. Infusions are water extracts that do not require either sophisticated equipment or expensive or scarce extractants. From a biopharmaceutical point of view, aqueous extracts provide good availability of drug...