Phytochemical screening and In vitro bioactivities of the various extracts of Carica papaya leaves available in Bangladesh
Journal Title: International Journal of Research in Pharmacology & Pharmacotherapeutics (IJRPP) - Year 2015, Vol 4, Issue 3
Abstract
AIM OF THE STUDY To evaluate the presence of different phyto constituents and investigate in vitro bioactivities of chloroform, methanol and aqueous extracts of Carica papaya leaves available in Bangladesh. MATERIALS AND METHODS Phytochemical screening was conducted using specific standard procedure. Antioxidant activity was evaluated using DPPH radical scavenging assay, determination of total phenolic content and total flavonoid content. Antibacterial and cytotoxic activities were studied using disc diffusion method and brine shrimp lethality bioassay respectively. RESULTS Results showed that the chloroform extract had higher antioxidant activity (IC50 value for DPPH was 55.202 μg/ml and total phenolic content and total flavonoid content was 127.8±1.131 in mg/g, Gallic acid equivalents and 110.499±1.65 in mg/g, Quercetin equivalents) compared to the methanol and aqueous extract. In antibacterial study, all the extracts showed mild to moderate activity with zone of inhibition ranging from 7 mm to 16 mm. In brine shrimp lethality bioassay, the LC50 values for chloroform, methanol and aqueous extracts were 17.142 μg/ml, 15.404 μg/ml and 18.126 μg/ml respectively which revealed very strong cytotoxic potential of the extracts. CONCLUSION The results indicate that C. papaya leaves could be a very potent source of natural radical scavenger and anticancer agent. Further studies are needed to be conducted to identify the compounds responsible for producing such bioactivities.
Authors and Affiliations
Nazia Hoque
Keywords
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