Phytochemical screening, antimicrobial and antioxidant efficacy of different extracts of Rumex dentatus L. - A locally used medicinal herb of Kashmir Himalaya
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2013, Vol 3, Issue 6
Abstract
Objective: To elucidate the antimicrobial and antioxidant activities of Rumex dentatus L. (R. dentatus) along with its phytochemical analysis. Methods: Agar disk diffusion method for antimicrobial activity and DPPH, riboflavin photooxidation, deoxyribose and lipid peroxidation assay for antioxidant activity. Results: The antimicrobial and antioxidant activities of different concentrations of five R. dentatus extracts were tested against different clinical bacterial strains (Shigella flexneri, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhimurium) and fungal strains (Aspergillus versicolor, Aspergillus flavus, Accremonium spp., Penicillium dimorphosporum, Candida albicans, Candida parapsilosis, and Candida kruesie). Among all extracts, the butanol extract showed strong antibacterial activity against Klebsiella pneumoniae (inhibition zone diameter of 20 mm) and aqueous extract showed no activity against any of the bacterial strains. While as in case of the fungal strains, the maximum antifungal activity was observed against Aspergillus flavus by aqueous extract. The antioxidant activity revealed that the extracts exhibited scavenging effect in concentration-dependent manner on superoxide anion radicals and hydroxyl radicals. The phytochemical tests carried out with the crude extracts of R. dentatus showed the presence of flavonoids, terpenoids, alkaloids, saponins, tannins, anthraquinones and cardiac glycosides in it. The total phenolic content of these extracts was estimated quantitatively from standard calibration curve of gallic acid and it varied from 145 µg/mg in butanol extract to 45 µg/mg in petroleum ether extract. Conclusions: It can be concluded that the plant has got a broad spectrum antimicrobial and antioxidant activity and could be used as a potential alternative for treating various diseases.
Mosquito larvicidal and biting deterrency activity of bud of Polianthes tuberosa plants extract against Anopheles stephensi and Culex quinquefasciatus
Objective: To evaluate the larvicide and biting deterrency activity of bud of Polianthes tuberosa (P. tuberosa) against Culex quinquefasciatus (Cx. quinquefasciatus) and Anopheles stephensi (An. stephensi). Methods: Crud...
Hospitalized pediatric antituberculosis drug induced hepatotoxicity: Experience of an Indonesian referral hospital
Objective: To determine the characteristics and risk factors of pediatric antituberculosis drug induced hepatotoxicity (ADIH) in Dr. Hasan Sadikin Hospital, a referral hospital in West Java, Indonesia. Methods: Medical r...
Evaluation of larvicidal activity of the aerial extracts of a medicinal plant, Ammannia baccifera (Linn) against two important species of mosquitoes, Aedes aegypti and Culex quinquefasciatus
Objective: To analyze the larvicidal effect of the aerial extracts of Ammannia baccifera on two important mosquito species, Aedes aegypti and Culex quinquefasciatus. Methods: The larval mortality of fourth instar larvae...
Molecular epidemiology and geographical distribution of Nosema ceranae in honeybees, Northern Thailand
Objective: To determine the contamination levels of Nosema ceranae in honeybees and its molecular linkages in different geographical areas of Northern Thailand. Methods: Seventy-eight apiaries in Northern Thailand were c...
Evaluation of the anticancer potentials of Origanum marjorana on fibrosarcoma (HT-1080) cell line
Objective: To evaluate the potential anticancer activity of Origanum majorana (marjoram) (O. marjorana) on the fibrosarcoma cancer cell line HT-1080 through various in-vitro assays. Methods: Crude extracts were prepared...