Phytochemical Screening, Mineral Content Analysis and Assessment of Antibacterial Activity of Methanol Extract of NewbouldialaevisLeaf
Journal Title: Saudi Journal of Pathology and Microbiology - Year 2017, Vol 2, Issue 6
Abstract
Abstract: This study dealt with the assessment of the phytochemicals, antibacterial and mineral analysis of methanol extract of leafNewbouldialaevis and its fractions (hexane, chloroform and ethyl acetate). Quantitative phytochemical screenings were done by various standard laboratory methods. Quantitative phytochemical screening was carried out using spectrophotometry method while mineral analysis was determined by atomic absorption spectrophotometric method. Antibacterial activity was investigated using agar well diffusion method.The result obtained from the phytochemical screening revealed the presence of alkaloids, phenols, oxalate, tannin, saponin among others. The antibacterial activity of Newbouldialaevisleaf extracts was tested on Escherichia coli, Staphylococcus aureus, Klebsiellia pneumonia, and Streptococcus pneumonia. Antibacterial effect of Newbouldialaevis leaf showed that only n-hexane fraction was capable of inhibiting all microorganisms used except Staphylococcus aureus in concentration dependent manner. Chloroform fraction was found to inhibit only E.coliat higher concentration (100-150 mg/ml) as indicated in the zone of inhibition, while it has no effect on other bacteria. Methanol extract showed inhibition activity only on S. aureus at 100-150 mg/ml. All the microorganisms showed resistance to the aqueous fraction of Newbouldialaevisleaf. Mineral analysis revealed the presence of Zn, Co, Cd, Cu, Ca, Fe and Mn.These results could suggest the promising chemopreventive use of Newbouldialaevisand some of its active principles in the treatment of infections. Keywords:Newbouldialaevis; phytochemical; mineral analysis; bacterial activity
Authors and Affiliations
SalemcityAanuoluwa James, Awe Sunday, Achem Jonah, Akor Palmer Ojonugwa, AbuhOjima Victoria
Keywords
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