Phytochemical Screening, Total Phenolic Content, Antioxidant and Cytotoxic Activity of Chromolaena laevigata on Human Tumor Cell Lines
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 21, Issue 1
Abstract
Aims: Cancer is the first cause of death in the Peruvian population; searching alternative treatments of medicinal plants constitute a promissory field to find new anticancer drugs. The main objective in this study was to evaluate the phytochemical screening, total phenolic content, antioxidant and cytotoxic activity of ethanol extract of Chromolaena laevigata (C. laevigata) on human tumor cell lines. Study Design: The fresh leaves of C. laevigata were soaked with ethanol followed by phytochemical screening using standard methods. Place and Duration of Study: Laboratory of Applied Chemistry, Faculty of Pharmacy and Biochemistry, Universidad Nacional San Luis Gonzaga de Ica, Ica, Peru; Laboratory “Abraham Vaisberg Wolach”, Universidad Peruana Cayetano Heredia, Lima, Peru. Methodology: Phytochemical screening was assessed by using chemical reactives. Total phenolic content (TPC) was developed using Folin Ciocalteu reactive and the antioxidant activity was determined against DPPH and ABTS radicals by spectrophotometry. The cytotoxic activity was determined on human tumor cell lines followed as: MCF-7, H-460, HT-29, M-14, K-562 and DU-145. Results: Phytochemical study confirmed flavonoids and phenolic compounds in ethanol extract. TPC resulted 45.21 ± 3.5 mg of gallic acid equivalent/g of dried extract. The highest antioxidant extract for DPPH and ABTS radical scavenging tests were IC50 = 11.66 ± 1.0 μg/mL, IC50= 12.45 ± 0.50 μg/mL respectively. Ethanolic extracts (μg/mL) showed a low cytotoxicity on human tumor cell lines (CI50 > 20 μg/mL) for DU-145, HT-29, MCF-7 and M-14. Whereas, for H-460, and K562 tumor cell lines showed high cytotoxicity. Conclusion: In our findings, C. laevigata demonstrated a high antioxidant and total phenolic content. The ethanol extract exhibited better cytotoxic effect compared with 5-FU. Hence, This medicinal plant could be effective to prevent chronical diseases as cancer and oxidative stress disorders.
Authors and Affiliations
Oscar Herrera-Calderon, Jorge Luis Arroyo-Acevedo, Juan Rojas-Armas, Victor Chumpitaz-Cerrate, Linder Figueroa-Salvador, Edwin Enciso-Roca, Johnny Aldo Tinco-Jayo
Keywords
Related Articles
- EP ID EP313538
- DOI 10.9734/ARRB/2017/37045
- Views 98
- Downloads 0
Biotic Factors Affecting the Abundance of Vascular Epiphytic Bromeliads Growing in Cloud Forest in Reserva Biologica Los Cedros, Ecuador
Aims: In this paper we investigate whether the abundance of epiphytic bromeliads growing in cloud forest was affected by: (1) phorophyte family; (2) phorophyte size; and (3) the abundance of other epiphytes. Study Design...
Therapeutic Management of Benign Prostatic Hyperplasia: From Synthetics to Naturals
Benign prostatic hyperplasia (BPH) is a common urological disorder of men with multi-factorial etiology, particularly affecting the lifestyle of elderly patients. Sex steroid hormones essential for the development and gr...
In vitro Microrhizome Formation in Kaempferia parviflora
Plantlets produced in vitro from rhizomes of Kaempferia parviflora were used as the source of explants for microrhizome development. Sixty percent of the plantlets formed microrhizomes in liquid medium supplemented with...
Pharmacognostic and Wound Healing Studies of the Leaves of Bassia eriophora (Family: Chenopodiaceae) on Albino Rats
Objective: To study the pharmacognostic profile and wound healing effect of aerial parts of Bassia eriophora (Family: Chenopodiaceae), a wild plant in Saudi Arabia, on albino rats. Methods: The microscopy, phytochemical...
Gallbladder Expression of Metallothionein I/II in Cholecystolithiasis. A Pilot Study
Aims: To investigate the expression of metallothioneins (MTs) and free zinc content in gallbladderassociated with cholecystolithiasis. Study Design: Gallbladder tissue samples were obtained from patients, subjected to la...