Phytochemical Studies and GC-MS Analysis of Chloroform Extract of the Leaves of Aspilia africana
Journal Title: Asian Journal of Physical and Chemical Sciences - Year 2017, Vol 4, Issue 3
Abstract
Aim: The aim of this research was extraction, phytochemical studies and GCMS analysis of Aspilia africana. Place and Duration of Study: This study was carried out at the University of Benin, Benin-City and University of Lagos Central Research Laboratory, Nigeria from January 2016 to September 2016. Methodology: 200 g of the pulverized plant sample was extracted with hexane, chloroform and methanol in this order for 8-12 hours. The extract was concentrated using a rotary evaporator to obtain the crude extract. The phytochemical test was carried out using standard methods. The GCMS was carried out using Agilent Technologies 7890A couple with Agilent Technologies 5975C VL MSD. The mobile phase is helium gas while the stationary phase was the column agilent technology HP5 MS with length 30m, internal diameter 0.320 mm and thickness 0.25 microns. The volume injected is 1 microlitre, oven initial temperature was 80°C to hold for 2 minutes. The mode was split less and scan range was 35-55. Results: The phytochemicals of the leaves of Aspilia africana were extracted with chloroform by Soxhlet method and analysed using GC-MS. Ten constituents were identified constituting 92.714% hydrocarbon. Caryophyllene (29.43%), 2-Carene (19.76%) and Germacrene D (33.3%) were the major components of the extract. The phytochemical analysis was carried out by standard methods. The results revealed the presence of a number of therapeutically important phytochemicals such as alkaloids, terpenoids, tannins, flavonoids, saponins and glycosides in the extract. Conclusion: The results of the phytochemical screening and GC-MS analysis indicate that the leaves of Aspilia africana are a rich source of a number of bioactive secondary metabolites which include alkaloids, tannins, saponins, flavonoid, glycosides and terpenoids. This justifies the use of the plant in the traditional practices of herbal medicine, especially in the treatment of microbe-induced disease conditions.
Authors and Affiliations
Ogbemudia Ruth Etiosa, Adeyemo John Akeem, Nwadozie Blessing Chika
Keywords
Related Articles
- EP ID EP338821
- DOI 10.9734/AJOPACS/2017/38663
- Views 84
- Downloads 0
Turbulent Thermochemical Non-equilibrium Reentry Flows with Magnetic Actuation in 2D – Seven Species
In this work, a study involving the Maciel scheme to solve the reactive Favre averaged Navier-Stokes equations, coupled with a turbulence model and the Maxwell equations is performed. The Favre averaged Navier-Stokes equ...
Nutrient X-Ray Energy Shift Studies in Wheat Plants and Soils
Aims: To check the effect of soil environment on chemistry of potassium and calcium nutrients in wheat plants with varying soil conditions. Study Design: Energy Dispersive X-ray Fluorescence (EDXRF) measurements on plan...
Dependence of Quantum Decoherence on Wavelengths of a Particle in One Dimensional Box
In this paper, de Broglies wave particle duality relation is applied to a particle in one dimensional box problem to find the constraint for quantum decoherence of this system. It is observed that, for a particle of mass...
Analysis of Tomato Drying by Using a Solar Tower Dryer in Natural Convection
This work presents an experimental study of the drying process of tomato in thin layer thanks to an indirect solar dryer operating in natural convection called "solar tower dryer". This experiment was performed under var...
Determination of the Formation Constant of Some Transition Metal Ion- histidine Complexes in Water and Water-dioxane and Evaluation of their Thermodynamic Parameters
Aims: The formation constants of histidine complexes of Fe (III), Co (II), Ni (II), Cu(II), Zn (II) and Pb (II) at temperatures of 25, 30, 37, 45°C were determined potentiometrically, utilizing modified Bjerrum's metho...