PIGMENTS OF RHODOPSEUDOMONAS YAVOROVII IMV B-7620
Journal Title: Мікробіологія і біотехнологія - Year 2018, Vol 0, Issue 1
Abstract
Aim. Determination of spectra of photosynthetic pigments of the purple nonsulfur bacteria R. yavorovii IMV В-7620. Methods. Bacteria were grown in liquid modified medium of ATCC No. 1449 and tryptic soy agar under anaerobic or aerobic conditions, respectively. Separation of pigments was conducted by using the system of high-performance liquid chromatography. Results. The photosynthetic purple non-sulfur bacteria R. yavorovii IMV B-7620 can grow both under anaerobic and aerobic conditions of cultivation. In extracts of bacteria R. yavorovii IMV B-7620 pigments with the use of high-performance liquid chromatography three homologous forms of bacteriochlorophyll a were determined which had absorption spectra at λ max =361, 605, 770 nm. In the process of separating carotenoids, lycopene (at λ max =446, 473, 504 nm) and anhydrorhodovibrin (at λ max =459, 485, 519 nm) were identified. Conclusions. In the process of one-step separation of pigment mixtures with the use of high-performance liquid chromatography, it has been shown that cells of purple non-sulfur bacteria R. yavorovii IMV B-7620 under anaerobic conditions of cultivation contain bacteriochlorophyll a, lycopene and anhydrorhodovibrin.
Authors and Affiliations
O. V. Tarabas, S. O. Hnatush, A. A. Halushka, O. M. Moroz
Keywords
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- EP ID EP396602
- DOI 10.18524/2307-4663.2018.1(41).120468
- Views 92
- Downloads 0
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