Piperine content variation in different Piper longum germplasms of North East India determined through RP-HPLC method
Journal Title: Journal of Applied and Natural Science - Year 2017, Vol 9, Issue 2
Abstract
The present experiment was conducted at Assam Agricultural University, Jorhat with ten accessions including check variety of Piper Longum germplasm collected from different states of North Eastern region during 2013-14 and 2014-15.The piperine, a major alkaloid used in different therapeutic treatment, per cent content was determined from dried plant materials. Deionised water was used throughout the experiment and the chromatographic separation was carried out in an isocratic elution mode on RP-18 column with 5μm particle size, 4.6mm internal diameter and 250mm length. The mobile phase was a mixture of methanol and water (80: 20). The solvent flow rate was 1.0 ml/minwith injection volume 20 µL. The photo diode array detector wavelength was set at 342 nm for the identification of piperine in all extracts. The per cent piperine content in extract was found to be 42.36 per cent in germplasm PLJ-30.The highest per cent piperine content in fruit was recorded in PLJ-11(7.85%) followed by germplasm PLJ-9 (7.64) and significantly superior over check variety“Viswam”(5.15%). The descending order of piperine content in fruits among germplasm was PLJ-11˃PLJ-09˃PLJ-30˃PLJ-17˃ PLJ-03˃ PLJ-19˃ check variety˃PLJ-01˃ PLJ-20 and PLJ-16. It can be revealed from the present experiment that piper germplasm with high piperine content has a great scope for commercial cultivation as alkaloid piperine has high demand in pharmaceutical use.
Authors and Affiliations
A. Khound, P. C. Barua, B. Saud, S. Kumar
Keywords
Related Articles
- EP ID EP286609
- DOI 10.31018/jans.v9i2.1304
- Views 96
- Downloads 0
G × E evaluation for feed barley genotypes evaluated in country by AMMI analysis
AMMI analysis of feed barley genotypes exhibited highly significant effects of environments, genotypes and interactions for both the years. The major portion of the total variance was described by the environmental effec...
Constraints analysis in adoption of best farm practices towards sequestration of greenhouse gases
The aim of this paper is to find out the major losses due to emission of green house gases (GHGs) and investigate the major constraints that are responsible for non-adoption of best farming practices to minimize the emis...
Quantification of crop weather relationship and the effect of different planting dates on growth and yield of potato cultivars in a sub-tropical environment at Hisar
Field experiments were carried out at research farm of Department of Agricultural Meteorology, CCSHAU, Hisar during Rabi seasons of 2016-17 to quantify crop weather relationship and the effect of different planting dates...
Antioxidant activity and production of secondary metabolites of adult plant and in vitro calli of Anodendron paniculatum
The members of the family Apocynaceae are the rich in alkaloids, terpinoids, flavonoids, phenolic compounds and hydrocarbons. Anodendron paniculatum Roxb. is an woody climber, the roots of which have been used to control...
Neuromuscular systems in the fifth instar larva of silkworm Bombyx mori (Lepidoptera: Bombycidae): II- Abdominal musculature and its innervation
The gross organisation of abdominal musculature and its innervation by segmental ganglionic nerves were studied in the fifth instar larva of Bombyx mori. The musculature comprises dorsal, ventral and lateral groups of mu...