Plant regeneration of Dioscorea cayenensis subsp. rotundata Poir cv. ‘Blanco de Guinea’ from somatics embryos
Journal Title: Biotecnología Vegetal - Year 2018, Vol 18, Issue 3
Abstract
Despite the progress made in the production of yam (Dioscorea spp.) seed by both conventional and biotechnological methods, the low disponibility of planting material with physiological and sanitary quality continues to limit the production of this crop on a large scale. Somatic embryogenesis is an alternative for the propagation of plant material and an auxiliary tool for its genetic improvement. The present work was carried out with the aim of to regenerate D. cayenensis subsp. rotundata Poir cultivar ‘Blanco de Guinea’ lants from somatic embryos. The effect of three culture media on the maturation of somatic embryos and different combinations of 6-BAP and kinetin in MS culture medium for germinating somatic embryos was evaluated. The highest percentage of mature somatic embryos (36.66%) was obtained in MS culture medium with the salts at 50% of their concentration and without plant growth regulators, which favored their subsequent germination (60%) in MS culture medium with kinetin (1.0 mg l -1 ) and 6-BAP (2.0 mg l -1 ). The germinated somatic embryos were characterized by the presence of open leaves, well-defined stems with a dark green coloration and small white roots, which they continued their differentiation until forming complete plants at 60 days of culture.
Authors and Affiliations
Dayana Rodríguez, Jorge López , Idalmis Bermúdez-Caraballoso , Nery Montano , Aymé Rayas , Milagros Basail , Arletys Santos, Yenisey Gutiérrez , Víctor Medero , Yoel Beovides
Keywords
Related Articles
- EP ID EP682979
- DOI -
- Views 134
- Downloads 1
Evaluation of the knowledge of a population about the risk in the case of plantain and banana transformed with genes for resistance to the black sigatoka
An increasing impact of plant biotechnology on society is present in all over the world. Scientific understanding should be developed on people so they can be able to make right personal decisions and participate in pub...
Use of AFLP markers for molecular characterization of two crops with agricultural interest
The AFLP technique is based on the selective amplification of restriction fragment by polymerase chain reaction (PCR) and consists of four basic steps; digestion with restriction enzymes of deoxyribonucleic acid (DNA),...
Protocol for the differentiation of potato genotypes byartificial inoculation of mycelial suspensions from Alternaria solani Sor. in bedseeds and field condition
Early blight resistance evaluation in potatoes is commonly done under natural infection conditions, nevertheless there exist some disadvantages such as time consuming procedure, intensive labors for plantation maintenan...
Somatic embryo formation from cotyledons immature in Phaseolus vulgaris cv. CIAP 7247
Common bean, like most of legumes, is recalcitrant to in vitro culture, so the establishment of a regeneration procedure via somatic embryogenesis in this specie has not been possible. Therefore, determining the effect...
Influence of growth regulators on callus formation of Phaseolus vulgaris L cv. CIAP 7247
The present work had as objective to achieve the formation of nodular calli in Phaseolus vulgaris L. cv. CIAP 7247 using several growth regulators in the culture medium. Cotyledons of mature seeds without coats were use...